LOCUS Exported 6162 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA5/FRT/TO V5 DNAJB1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6162) AUTHORS Hageman J, Kampinga HH TITLE Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. JOURNAL Cell Stress Chaperones. 2008 Aug 7. ():. PUBMED 18686016 REFERENCE 2 (bases 1 to 6162) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6162 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 820..838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 869..893 /label=LNCX /note="Human CMV promoter, forward primer" CDS 996..1037 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(2114..2131) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2120..2344 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(2477..2496) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 2628..2675 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 2683..3705 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 3835..3956 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3872..3891) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3926..3945 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4005..4021) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4005..4021) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4018..4040) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4029..4045 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4053..4083) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4098..4119 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4236..4253) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4407..4995) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4487..4506) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5166..6026) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5789..5808 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6027..6131) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttgaattca ccatgggtaa gcctatccct aaccctctcc 1021 tcggtctcga ttctacggga tccaccatgg gtaaagacta ctaccagacg ttgggcctgg 1081 cccgcggcgc gtcggacgag gagatcaagc gggcctaccg ccgccaggcg ctgcgctacc 1141 acccggacaa gaacaaggag cccggcgccg aggagaagtt caaggagatc gctgaggcct 1201 acgacgtgct cagcgacccg cgcaagcgcg agatcttcga ccgctacggg gaggaaggcc 1261 taaaggggag tggccccagt ggcggtagcg gcggtggtgc caatggtacc tctttcagct 1321 acacattcca tggagaccct catgccatgt ttgctgagtt cttcggtggc agaaatccct 1381 ttgacacctt ttttgggcag cggaacgggg aggaaggcat ggacattgat gacccattct 1441 ctggcttccc tatgggcatg ggtggcttca ccaacgtgaa ctttggccgc tcccgctctg 1501 cccaagagcc cgcccgaaag aagcaagatc ccccagtcac ccacgacctt cgagtctccc 1561 ttgaagagat ctacagcggc tgtaccaaga agatgaaaat ctcccacaag cggctaaacc 1621 ccgacggaaa gagcattcga aacgaagaca aaatattgac catcgaagtg aagaaggggt 1681 ggaaagaagg aaccaaaatc actttcccca aggaaggaga ccagacctcc aacaacattc 1741 cagctgatat cgtctttgtt ttaaaggaca agccccacaa tatctttaag agagatggct 1801 ctgatgtcat ttatcctgcc aggatcagcc tccgggaggc tctgtgtggc tgcacagtga 1861 acgtccccac tctggacggc aggacgatac ccgtcgtatt caaagatgtt atcaggcctg 1921 gcatgcggcg aaaagttcct ggagaaggcc tccccctccc caaaacaccc gagaaacgtg 1981 gggacctcat tattgagttt gaagtgatct tccccgaaag gattccccag acatcaagaa 2041 ccgtacttga gcaggttctt ccaatatagg cggccgctcg agtctagagg gcccgtttaa 2101 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 2161 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 2221 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 2281 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 2341 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 2401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 2461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 2521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 2581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc 2641 cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc 2701 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag 2761 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc 2821 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt 2881 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg 2941 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa 3001 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt 3061 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg 3121 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac 3181 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac 3241 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac 3301 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac 3361 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc 3421 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc 3481 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct 3541 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca 3601 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat 3661 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat 3721 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 3781 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 3841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 3901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 3961 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 4021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 4081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 4141 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 4201 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 4261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 4321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 4381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 4441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 4501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 4561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 4621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 4681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 4741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 4801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 4861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 4921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 4981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 5281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 5341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 5401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 5461 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 5521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 5581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 5641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 5701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 5761 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 5821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 5881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 5941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //