LOCUS Exported 5868 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA5/FRT/TO V5 DNAJB3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5868) AUTHORS Hageman J, Kampinga HH TITLE Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. JOURNAL Cell Stress Chaperones. 2008 Aug 7. ():. PUBMED 18686016 REFERENCE 2 (bases 1 to 5868) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5868 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 820..838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 869..893 /label=LNCX /note="Human CMV promoter, forward primer" CDS 996..1037 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(1820..1837) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1826..2050 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(2183..2202) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 2334..2381 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 2389..3411 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 3541..3662 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3578..3597) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3632..3651 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3711..3727) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3711..3727) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3724..3746) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3735..3751 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3759..3789) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3804..3825 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3942..3959) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4113..4701) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4193..4212) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4872..5732) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5495..5514 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5733..5837) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttgaattca ccatgggtaa gcctatccct aaccctctcc 1021 tcggtctcga ttctacggga tccaccatgg tggactacta cgaggtgctg ggcgtgccgc 1081 ggcaggcctc agccgaggcc atccgcaagg cgtaccgcaa gcttgcgctc aagtggcacc 1141 cggacaagaa ccccgagcac aaggaggagg ccgagaggcg gttcaagcag gtggcgcagg 1201 cctacgaggt cttatcggac gtccgcaagc gcgaggtgta cgaccgctgc ggcgaagtgg 1261 gcgaggtggg cggaggcggc gcggcgggca gcccgttcca cgatgccttc cagtacgtct 1321 tctccttccg agaccccgcg gaggtcttca gggagttctt cgggggccac gaccctttct 1381 ccttcgactt cttcggggga gacccccttg agaacttttt tggggatcgg aggagcaccc 1441 gtggaagcag aagccgaggg gctgtaccct tctctacctc ctttaccgaa ttcccaggat 1501 ttgggggtgg cttcgcttcg ttagatactg gattcacatc cttcggttct ccaggaaact 1561 cgggcctttc ttctttctcc atgtcttgcg gcggtggggc ggcaggcaac tacaagtcgg 1621 tgtcaacctc caccgaaata attaatggca aaaaaatcac caccaagaga atcgttgaga 1681 atggtcaaga aagggtggaa gtggaagaag atggagagtt aaagtccctg ataattaatg 1741 gcagagagca gctgcttcgc atcaatactc agtaagcggc cgctcgagtc tagagggccc 1801 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 1861 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 1921 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 1981 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 2041 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 2101 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 2161 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 2221 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 2281 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc 2341 ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa 2401 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg 2461 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga 2521 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg 2581 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag 2641 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa 2701 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc 2761 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact 2821 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg 2881 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc 2941 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg 3001 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc 3061 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag 3121 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat 3181 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat 3241 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg 3301 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc 3361 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta 3421 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 3481 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 3541 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 3601 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 3661 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 3721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 3781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 3841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 3901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 3961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 4201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 4261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 4321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 4381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 4441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 4501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 4561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 4621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 4681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 4741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 4801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 4861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 4921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 4981 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5161 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 5221 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 5281 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 5341 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 5401 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 5461 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 5521 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 5581 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 5641 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 5701 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 5761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 5821 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc //