LOCUS Exported 3133 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION ORF encoding p38 KTR mClover flanked by Gateway sequences. ACCESSION . VERSION . KEYWORDS pENTR-p38KTRClover SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3133) AUTHORS Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW TITLE High-sensitivity measurements of multiple kinase activities in live single cells. JOURNAL Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039. PUBMED 24949979 REFERENCE 2 (bases 1 to 3133) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3133 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 103..189 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 281..308 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind 316..339 /label=pENTR-F /note="pENTR vectors, forward primer" protein_bind 358..457 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(697..718) /label=EGFP-N /note="EGFP, reverse primer" primer_bind 1305..1326 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(1362..1461) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1573..1592) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1584..2393 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1651..1670) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2483..3071 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2972..2991 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgcgtaa gccagatctc 481 cgcgttctta tcccacctgg cagcaagaac acgatgccat cagtgaatca aaggataaat 541 aactccaagc gctcggccgg tctcaacgaa gacgagtcgc catctaagaa gctggcgtcg 601 ccggaggtgt cgtcaaggct ggagcgcctg accctccagt ccagcactag tatggtgagc 661 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 721 aacggccaca agttcagcgt ccgcggcgag ggcgagggcg atgccaccaa cggcaagctg 781 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 841 accttcggct acggcgtggc ctgcttcagc cgctaccccg accacatgaa gcagcacgac 901 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctc tttcaaggac 961 gacggtacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 1021 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 1081 tacaacttca acagccacaa cgtctatatc acggccgaca agcagaagaa cggcatcaag 1141 gctaacttca agatccgcca caacgttgag gacggcagcg tgcagctcgc cgaccactac 1201 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 1261 catcagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 1321 ttcgtgaccg ccgcctaagc ggccgcactc gagatatcta gacccagctt tcttgtacaa 1381 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag 1441 tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct 1501 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 1561 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat 1621 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc 1681 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga 1741 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc 1801 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat 1861 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg 1921 attcaggtga aaatattgtt gatgcgctgg cagtgtccct gcgccggttg cattcgattc 1981 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac 2041 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg 2101 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca 2161 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta 2221 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact 2281 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata 2341 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat 2401 tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagacccgg 2461 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 2521 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 2581 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 2641 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 2701 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 2761 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 2821 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 2881 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 2941 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 3001 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 3061 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 3121 ttgctcacat gtt //