LOCUS Exported 6218 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pcDNA5/FRT/TO V5 DNAJB5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6218) AUTHORS Hageman J, Kampinga HH TITLE Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. JOURNAL Cell Stress Chaperones. 2008 Aug 7. ():. PUBMED 18686016 REFERENCE 2 (bases 1 to 6218) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6218 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 820..838 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 841..859 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 869..893 /label=LNCX /note="Human CMV promoter, forward primer" CDS 996..1037 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(2170..2187) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2176..2400 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(2533..2552) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 2684..2731 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 2739..3761 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 3891..4012 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3928..3947) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3982..4001 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4061..4077) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4061..4077) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4074..4096) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4085..4101 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4109..4139) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4154..4175 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4292..4309) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4463..5051) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4543..4562) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5222..6082) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5845..5864 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6083..6187) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc 841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga 901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga 961 ctctagcgtt taaacttaag cttgaattca ccatgggtaa gcctatccct aaccctctcc 1021 tcggtctcga ttctacggga tccactagtc cagtgtggtg gaattctgca gatatccagc 1081 acagtggcgg ccgcaccatg ggaaaagatt attacaagat tcttgggatc ccatcggggg 1141 ccaacgagga tgagatcaag aaagcctacc ggaagatggc cttgaagtac cacccagaca 1201 agaataaaga acccaacgct gaggagaagt ttaaggagat tgcagaggcc tatgatgtgc 1261 taagtgaccc caagaaacgg ggcctgtatg accagtatgg ggaggaaggc ctgaagaccg 1321 gcggtggcac atcaggtggc tccagtggct cctttcacta cacctttcat ggggaccccc 1381 atgccacctt tgcctccttc tttggtggct ccaacccctt cgatatcttc tttgccagca 1441 gccgctccac tcggcccttc agtggctttg acccagatga catggatgtg gatgaagatg 1501 aggacccatt tggcgctttc ggccgttttg gcttcaatgg gctgagtagg ggtccaaggc 1561 gagccccaga accactgtac cctcggcgca aggtgcagga ccccccagtg gtgcacgagc 1621 tgcgggtgtc cctggaggag atctaccatg gctccaccaa gcgcatgaag atcacaaggc 1681 gtcgcctcaa ccctgatggg cgaactgtgc gcaccgagga caagatcctg cacatagtca 1741 tcaagcgtgg ctggaaggaa ggcaccaaga tcaccttccc caaagaaggc gacgccacac 1801 ctgacaacat ccctgctgac atcgtctttg tgctcaaaga caagccccat gcacacttcc 1861 gccgagatgg caccaacgtg ctctacagtg ccctgatcag cctcaaggag gcgctgtgtg 1921 gctgcactgt gaacattccc actatcgacg gccgagtgat ccctttgccc tgcaatgatg 1981 tcatcaagcc aggcaccgtg aagagactcc gtggggaggg ccttcccttc cccaaagtgc 2041 caactcagcg aggagacctc attgttgagt tcaaagttcg cttcccagac agattaacac 2101 cacagacaag acagatcctt aagcagcacc taccctgttc ctaggggccc gtttaaaccc 2161 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 2221 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 2281 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 2341 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 2401 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 2461 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 2521 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 2581 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 2641 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa 2701 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga 2761 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct 2821 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc 2881 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat 2941 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct 3001 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc 3061 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc 3121 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg 3181 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca 3241 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc 3301 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg 3361 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg 3421 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact 3481 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca 3541 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg 3601 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa 3661 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg 3721 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg 3781 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 3841 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 3901 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 3961 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 4021 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 4081 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4141 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 4201 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4261 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4861 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 4921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5041 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5161 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5221 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5281 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5341 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5401 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 5461 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5521 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5581 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5641 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5701 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5761 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5821 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5881 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5941 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 6001 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 6061 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 6121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 6181 ttccgcgcac atttccccga aaagtgccac ctgacgtc //