LOCUS       Exported               10747 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Fluorescent membrane voltage sensor.
ACCESSION   .
VERSION     .
KEYWORDS    FCK-QuasAr2-mRuby2 eFRET
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   Zou P, Zhao Y, Douglass AD, Hochbaum DR, Brinks D, Werley CA, 
            Harrison DJ, Campbell RE, Cohen AE
  TITLE     Bright and fast multicoloured voltage reporters via electrochromic 
            FRET.
  JOURNAL   Nat Commun. 2014 Aug 13;5:4625. doi: 10.1038/ncomms5625.
  PUBMED    25118186
REFERENCE   2  (bases 1 to 10747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10747
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    5439..6027
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5492..5512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5910..5921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6030..6046)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6031..6047)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6552..6732
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6758..6775)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6764..6988
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7034..7462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7121..7140)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7331..7352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7471..7491)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7476..7805
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7656..7791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7718..7737
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7853..7900
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7919..8293
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8423..8544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8460..8479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8514..8533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8593..8609)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8593..8609)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8606..8628)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8617..8633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8641..8671)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8686..8707
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8824..8841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8995..9583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9075..9094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9754..10614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10377..10396
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10615..10719)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatcca
     3901 ccatggtaag tatcgctctg caggctggtt acgacctact gggtgacggc agacctgaaa
     3961 ccctgtggct gggcatcggc actctgctga tgctgattgg aaccttctac tttctggtcc
     4021 gcggatgggg agtcaccgat aaggatgccc gggaatatta cgctgtgact atcctggtgt
     4081 ccggaatcgc atccgccgca tatctgtcta tgttctttgg tatcgggctt actgaggtgt
     4141 ccgtcggggg cgaaatgttg gatatctatt atgccaggta cgcccagtgg ctgtttacca
     4201 ccccacttct gctgctgcac ctggcccttc tcgctaaggt ggatcgggtg accatcggca
     4261 ccctggtggg tgtggacgcc ctgatgatcg tcactggcct catcggagcc ttgagccaca
     4321 cggccatagc cagatacagt tggtggttgt tctctacaat ttgcatgata gtggtgctct
     4381 atgttctggc tacatccctg cgatctgctg caaaggagcg gggccccgag gtggcatcta
     4441 cctttaacac cctgacagct ctggtcttgg tgctgtggac cgcttaccct atcctgtgga
     4501 tcataggcac tgagggcgct ggcgtggtgg gcctgggcat cgaaactctg ctgtttatgg
     4561 tgttggacgt gactgccaag gtcggctttg gctttatcct gttgagatcc cgggctattc
     4621 tgggcgacac cgagttgagg atcaaggaaa atatgcgtat gaaggtggtc atggaaggtt
     4681 cggtcaacgg ccaccaattc aaatgcacag gtgaaggaga aggcaatccg tacatgggaa
     4741 ctcaaaccat gaggatcaaa gtcatcgagg gaggacccct gccatttgcc tttgacattc
     4801 ttgccacgtc gttcatgtat ggcagccgta cttttatcaa gtacccgaaa ggcattcctg
     4861 atttctttaa acagtccttt cctgagggtt ttacttggga aagagttacg agatacgaag
     4921 atggtggagt cgtcaccgtc atgcaggaca ccagccttga ggatggctgt ctcgtttacc
     4981 acgtccaagt cagaggggta aactttccct ccaatggtcc cgtgatgcag aagaagacca
     5041 agggttggga gcctaataca gagatgatgt atccagcaga tggtggtctg aggggataca
     5101 ctcatatggc actgaaagtt gatggtggtg gccatctgtc ttgctctttc gtaacaactt
     5161 acaggtcaaa aaagaccgtc gggaacatca agatgcccgg tatccatgcc gttgatcacc
     5221 gcctggaaag gttagaggaa agtgacaatg aaatgttcgt agtacaacgc gaacacgcag
     5281 ttgccaagtt cgccgggctt ggtggtggca tggacgagct gtacaagagt agaatcacaa
     5341 gcgaaggcga gtacatcccc ctggatcaaa tagacataaa tgtaggtgga ttttgttatg
     5401 agaatgaagt ataagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     5461 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5521 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5581 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5641 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5701 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5761 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5821 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5881 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5941 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6001 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     6061 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6121 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6181 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6241 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6301 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     6361 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     6421 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6481 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     6541 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6601 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6661 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6721 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     6781 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     6841 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     6901 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     6961 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     7021 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7081 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7141 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7201 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7261 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7321 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7381 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7441 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     7501 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7561 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7621 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7681 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7741 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     7801 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     7861 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     7921 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     7981 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     8041 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     8101 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     8161 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     8221 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     8281 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     8341 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8401 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8521 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8581 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8641 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8701 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8761 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8821 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8881 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8941 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9421 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10621 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10681 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10741 acctgac
//
