LOCUS Exported 14349 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle. ACCESSION . VERSION . KEYWORDS p201N Cas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14349) AUTHORS Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA. TITLE Targeted genome modifications in soybean with CRISPR/Cas9. JOURNAL BMC Biotechnology. 2015;15:16 REFERENCE 2 (bases 1 to 14349) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14349 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1187..1816 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2250..3317 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin 3383..3577 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(3813..3835) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 3921..4061 /label=bom /note="basis of mobility region from pBR322" primer_bind 3976..3995 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(4076..4093) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4247..4835) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4327..4346) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4930..5745) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 5653..5672 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 5737..5756 /label=pENTR-R /note="pENTR vectors, reverse primer" misc_feature 6038..6062 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" protein_bind 6239..6260 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6275..6305 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6313..6329 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6318..6340 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6337..6353 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6337..6353 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS complement(6841..7635) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6952..6971) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 7562..7581 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter 8781..9457 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" primer_bind 9394..9414 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 9503..13606 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 13619..13639 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 13706..13958 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(14000..14017) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(14000..14016) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(14009..14031) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 14212..14236 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag 61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg 121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac 181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc 241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc 301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc 361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg 421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc 481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg 541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc 601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg 661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg 721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa 781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc 841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg 901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg 961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg 1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc 1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg 1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc 1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc 1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg 1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac 1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc 1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt 1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg 1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg 1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga 1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct 1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa 1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta 1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac 1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag 1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag 2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg 2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg 2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg 2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg 2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc 2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga 3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc 3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg caggatcatg 4921 aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 4981 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 5041 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 5101 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 5161 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc 5221 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 5281 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 5341 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 5401 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa 5461 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 5521 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 5581 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga 5641 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt 5701 taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 5761 actgtttatg taagcagaca gttttattgt tcatgatctg gatcacaggc agcaacgctc 5821 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca 5881 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa 5941 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg 6001 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa 6061 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta 6121 acgccgaatt gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 6181 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 6241 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 6301 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta 6361 cgaattcctt gactagaggg taggcgcggc ttaattaagc cgcgccgtac cccaatagtc 6421 tcgacagaca catagcacct aacattattg cttaaattta ctattttttt actatattat 6481 actcaaccca atgagcataa agactgtaaa atctcaaatt cctgagaagc atatttatcg 6541 atcccacaga cttgatagtt ccataatcca tacgctgcag ccaaattgct agtgtgttga 6601 acatttaaca cgtagagaac tagaaaagat ataaaactaa gattgatatc caaaatagac 6661 gagaacaata agcaaaaact cttagttttg aaataaatca acaatcccga gggttgtcac 6721 atacatcaaa aacgaaaatc catatagcaa aaaaaactct aaattaccgt tcgacaaaaa 6781 gagaaaactg ataggacatt tgctaaacat taaaatcaaa atttggatca agccgaattc 6841 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat 6901 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg 6961 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa 7021 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac 7081 gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc 7141 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt 7201 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag 7261 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg 7321 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc 7381 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg 7441 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac 7501 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg 7561 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc 7621 atcttgttca atcatctcga gacgtaccgc gcgatgcatt cgaagatcga tccttcgcct 7681 ggaggagaga aatcagtggc gctgcggctt ttagggtttc tttgttgatg gaatgagagt 7741 gtaagctctg ccagtgccac tttattaggg ttttacaagc ccttttcttc gtaattgggc 7801 ctgacatttt gtgccacttg ggcctttaga gatgaaaatg tatattgggc ttaagttgac 7861 ttgaaggata aattagttta ggatattacg ttttttatga gaattggtgt gtcggataca 7921 tgtatatgat gcattcaaat atatgtattc tagatacatt taagtttaga tacaatctaa 7981 aatgtgtctt taattacagg actgtaacta aaatacttaa tgtaagaaga atattactcc 8041 tttaatagct tttgagtata tctagtctaa catcttttaa aaaagtctaa tttctttcat 8101 ttatttttcg agcaatagca aagtgcataa ttattttttt cttctagaaa ttcagatttg 8161 tttctctaaa ttttgagatt cttttctcaa ttttgtatgt ctagagaaca atgtgtattt 8221 ttcactctag ttggttgtta ctttgttgaa tgttctgata aaagtatatt gttatttctg 8281 aagtagatat aaaccttcat ttggaaatta tacataaatc aaaatcgtta attatctaga 8341 tcaagatata tgcccttttc ctaatgtatt tgatacatgc acctaatttc actagatgta 8401 tcttttctat tttttaaatt atgaatagtt aattttttcc atatgtgtat ttgatacata 8461 cttcatgact ttaaaaaatt aattatatac cagatatatg tatttaaaat ttgttatgta 8521 tttaaagtat gtatatgatt attcgatatt aatctcttcg atgaaattta aatcgataac 8581 tatgtgcttt ggatcgatct gcccactagt gagtcgtatt acaattcatg actctcttaa 8641 ggtagccaaa atcgatatct agggataaca gggtaatcga tcactagaat tcggcgcgcc 8701 gggcccaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc 8761 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc 8821 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc 8881 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac 8941 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca 9001 accacgtctt caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta 9061 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca 9121 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa 9181 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc 9241 tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag 9301 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat 9361 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat 9421 ataaggaagt tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa 9481 tctatctctc tcgaccgcta gcatggacaa gaagtactcc attgggctcg atatcggcac 9541 aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa 9601 agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt 9661 cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac 9721 ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt 9781 ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca 9841 cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc 9901 aaccatatat catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt 9961 gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga 10021 cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa 10081 tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag 10141 cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa 10201 gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa 10261 atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga 10321 tgatctcgac aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc 10381 aaagaacctg tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac 10441 caaagctccg ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac 10501 tttgctgaag gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga 10561 tcagtctaaa aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta 10621 caaatttatt aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct 10681 taacagagaa gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca 10741 gattcacctg ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt 10801 gaaagataac agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg 10861 ccccctcgcc cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat 10921 cactccctgg aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga 10981 aaggatgact aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct 11041 gctgtacgag tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg 11101 gatgagaaag ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt 11161 caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga 11221 atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac 11281 gtatcacgat ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga 11341 ggacattctt gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga 11401 agaacgcttg aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag 11461 gcgccgatat acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa 11521 gcagagtgga aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt 11581 catgcagttg atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt 11641 ttctggccag ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat 11701 caaaaaggga atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag 11761 gcataagccc gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg 11821 acagaagaac agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc 11881 ccaaatcctt aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct 11941 gtactacctg cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct 12001 ctccgactac gacgtggatc atatcgtgcc ccagtctttt ctcaaagatg attctattga 12061 taataaagtg ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga 12121 agaagttgtc aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac 12181 acaacggaag ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa 12241 agccggcttc atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca 12301 aattctcgat tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt 12361 gaaagttatt actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta 12421 taaggtgaga gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt 12481 aggcactgca cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta 12541 taaagtgtac gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac 12601 cgctaagtac ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc 12661 caatggagag attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt 12721 gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa 12781 catcgttaaa aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa 12841 aaggaacagc gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg 12901 attcgattct cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa 12961 gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag 13021 cttcgaaaaa aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga 13081 cctcatcatt aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat 13141 gctcgctagt gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt 13201 taatttcttg tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga 13261 gcagaagcag ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat 13321 aagcgaattc tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc 13381 ttacaataag cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt 13441 tactctgacc aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag 13501 aaagcggtac acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac 13561 ggggctctat gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgaccc 13621 caagaagaag aggaaggtgt gaccgcggat atctgcagaa gcttacgcgt cgacgtctcg 13681 aggccggccg agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag 13741 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa 13801 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag 13861 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga 13921 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatat cgtagggata 13981 acagggtaat aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 14041 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 14101 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgagctt 14161 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat 14221 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg 14281 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct 14341 cgggatcaa //