LOCUS       Exported                 489 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  Green fluorescent indicator for calcium imaging in the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV CEPIA2mt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    98..322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     311..328
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(364..393)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac
       61 cccctagagc cccagctggt tctttccgcc tcagaagcca tagagcccgg gccatcccca
      121 gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc
      181 agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg
      241 acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat
      301 ggctggcaac tagaaggcac agtcgaggct gatcagcgag cttctagact atgcggcccc
      361 attcagatcc tcttctgaga tgagtttttg ttctgcggcc gccttcgctg tcatcatttg
      421 tacaaactct tcgtagttta cctgaccatc cccatcgatg tctgcttccc tgatcatttc
      481 atcaacctc
//
