LOCUS Exported 17552 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION helper plasmid for bacterial conjugal DNA transfer. ACCESSION . VERSION . KEYWORDS pRL623 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 17552) AUTHORS Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP TITLE Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. JOURNAL J Bacteriol. 1997 Mar;179(6):1998-2005. PUBMED 9068647 REFERENCE 2 (bases 1 to 17552) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..17552 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 218..237 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 10657..10676 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10897..10914 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11155..11177 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 ctgcaggtcg actctagagt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc 61 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga 121 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc 181 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc 241 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg 301 aactgaacac gtcgcgtgac cgtgaaaaca ccttcgaacc gcagttgatt aagaagcacc 361 agacgcgcat tacgcagatg gacagccaga ttttatccct ctacgccaaa gatatgacta 421 cccgcgaaat tgtcgccacc ttcaaagaga tgtacgacgc agatgtgtca cccacgctga 481 tatgtaaagt cactgatgcg gtcaaagagc aggttgcaga atggcaaaat cgacctctgg 541 atgcactgta tcccattgtt taccttgact gtatcgtggt gaaagttcgt caaggtggca 601 ccgtaattaa caaagcggtg ttccttgcac tcggtattaa tactggaggc cagaaagagt 661 tgctgggcat gtggctggcc gagaacgaag gggcgaagtt ctggctgggg gggctgacag 721 agcttaagaa tcgggggctt caggatatcc ttattgcctg catggacggt ctgaaaggct 781 ttccggatgc gataaacagc gtgtatccgt agacgcacat ccagttgtgc atcattcata 841 tggtgcgcaa cagcctgaaa tacgtatcgt ggaaggacta caaagccgtc actggcgggt 901 taaaaacggt gtatcaggcg ccaacagaag aggcggcgct agtggcaccg gagcagttcc 961 cgaatgtctg ggacgataaa tacccgcaaa tcagcaaaag ctggcgtgcg cactgggaaa 1021 atatcaacac gttcttcgct attcgcccga tatccgcaag gccatctaca ccacgaacgc 1081 tatcgaatcg ctgaacagcg tgatccgtgc ggccattaag aagcgtaaag tgttcccgac 1141 tgacgactcg ctgcggaagg tcatttctct ggcgatcaag gatgcatcaa aaaaatggaa 1201 tatgccaatc cagagctggc ggctggcgat gagtcgcttt attatcgagt tcggtgaccg 1261 cctgagcgat cacctttaat atggcagcaa ttacacagaa ttatttacag ggtctatctt 1321 ttatttatct tatctaaaag atgtaccaca gatttgccaa cagcaaatga cagattaaca 1381 ggaacagcgt ttcctatttg cttatatttc gctgacattg ggccttcaaa tacccattca 1441 tcggggaagg tctgtattct tgcatactca cgcacagtta atggtcttgt ttcttctggg 1501 tggcaacgct ctgtttgttt ctgtgctggg gcgcatgtta atgttaggct tggttcatcc 1561 catgacaaac gacgagccat accagttttg cccccaccta agtaaaaact cttgagcatg 1621 tattcttttt gaatatcttc aggaagatct ctccagtagc caccgggagg aaccatactt 1681 aggatctctg cttttctttt gggatatttt tgtccttcag attctggcac gtcgctatca 1741 tacagctctc ccttttttaa tgcatcttta agggttaata ttttattgta agaagaaggc 1801 cactcataat cgatgccatc agcaagatca tttcttacag ctacaatgat caaacgctcg 1861 cgtttttgcg gcactttgta gaaaatagcc ttaagcactc ttggctcaaa taaagtgtag 1921 cccaagtctg tgataatatt ttttattgtt tctaaagttc gtccagcatc atgatttagc 1981 aaccctcgaa cattctctgc taaaagaact ttcggattga tttctttagc ggctcgggcg 2041 aattcaaaga aaagggtgcc ccgtgtatct tcaaaaccaa gttttttgcc tgcataagag 2101 aatgcctggc aaggaaagcc accagccagc acatcaacgg tattcctata aggggtgaag 2161 tctacttggc tcacatcacc ttcaaccaca ttccattcag gcctattttt tcgtaacgtc 2221 ttacaagcat gggagtcaat ttcatttagt aaaacagatt ttaaaccggc tttttctaaa 2281 cctaaagcca tcccccctgc gccagcaaat aactctaata cagtataaac attattgctt 2341 attttagtct catcaggcca ctggctttta aataaaaact gagcttgttc aaaattttta 2401 agttgctctt ttttataaaa tcgatagttg ttttcgtcat ttctttgaga aactaatttt 2461 ccagcagtat cccaacgcct taaagtttct tttgaaacgc ccaaaatgtc tgcaacttct 2521 gaaagtgaaa actcttcctt taacatattt agtaaccatg ccaacattag tcatggttac 2581 tactgtatgg gtttacatgt gctttgtcaa acattgatca tggttatcag ccgtaagcga 2641 gcccaaaata cttaataaaa ctatctttat cagggatgga aaaaccttgc tgcccaagac 2701 gttcgctcaa aatatactca attgcgtgag ggagagcact atggagttct tgcagagcat 2761 ctggtctgcc agtgacaagc tcatagaaac tcgcaccatc aatgattcta atgttagggt 2821 tcgaaggaca cagagtacca ctacctttat tggaaggagt aaaggggctg ttatatctga 2881 taggtttacg agggattata ttaacaaagt acgcacagta atccttaaat cggctatgtt 2941 tcggtgatac caactcatct aagcctttat atttatctgc taaatccccg ccagtaaccg 3001 ttgagtattt atttttcacc tcagcgatga ttcttctttc ttcgttgaga agatccacag 3061 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag 3121 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg 3181 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac 3241 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag 3301 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact 3361 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttctagagga tccccccttt 3421 caatttggct tactcggatc tttaatgctc ttgcctgctc aacaaatggt gttgcttttc 3481 gatttctttc taaagctaaa gcaacaaatc ctgctcggac ttcttgatat ggagtcacaa 3541 gatcatcatt gctttgaata tgatattgat atggcactag ggttttctcc aaacatacac 3601 acatttgcga agtgtctttc ttccatgtgt ccccatttgt tggctactgt tacctttgcc 3661 agtaggtaag acaagaattt tctccacatt aaaaccaatt tcttctgcaa tattagaaag 3721 aattaaatca accgaaatat caatacctgc atagcgaaca ttatcattta ccataaataa 3781 aggtgagcca ttttttaaaa ctctaaagca ttctataata acgcaagcca tttcatagaa 3841 atatcctttt atcatacgag gaataccgtt attattaagt ttttttcttt caagctctcg 3901 ctcaagaaag cgcaagatac tttgtaacaa ttcttgttta tcaagtatgc gtaatgcttc 3961 ctgccaattg tgaataagag acttttcttt gttttcaaca gtacaactca gcatatcttg 4021 cctaagttgt actatatctc tttctcccac acctaataga gctaattcta gagcgtatgt 4081 acgtgtatag tcataacgat tacaataggg tggagaggta atgatacagt cgtaaaaatc 4141 ttgctcaaat tcaggcagaa ttttaagaca tgaagcatta aataaattaa ttccacttct 4201 ttgagtccca taacaaaata atgtattact tattaagtca aaggaatcat tcaaaattaa 4261 ttttatttgc tcagtaattg cttcatcgaa atccagaatt ttacctttat caaatttatc 4321 tgatccactt ttcctaaatg ctcttttatc ccaacgtaga tactgtccat ctttacgggt 4381 atagctgata gattcaagaa tacacaatag agcaaaacgg agaacttgtt tcacaagaat 4441 attctctttt tctatagaaa ataaaaatct ctctattgat gcttctgttt caggaggata 4501 agctccgtca gtaattctta aacgattaag atattttcta ttattatgct gattccaagg 4561 tttttgtttg taccattcaa tcaatctcaa aaaatcttct cgacggaatt gtcgctggat 4621 tattttcctt acttcaataa tctcttgacc aataggtaac acttctatac catctgcttg 4681 aataccggca tcactacagg caaatagcat tgttcctgct cctgcgaagg ggtcaagaat 4741 cctctcaaat gattttttct cgtactcgaa tattaactga tttaccaaat caactgaaaa 4801 agcttcttta tatttaaacc agcgatactg aggttttgac ttattagctt gaaaactaac 4861 tatttttcga cttaaacttt catgcaatcg catcttatct ctaaaacgtt gctcaagtgc 4921 tttattgaat cgagaaattt cttgtacaaa ggaatcactc tcttgatcaa gatcagtcgg 4981 gtcagtcttt atttctattg gactctcaag ctcaaatgaa gtcattttat atttttcttg 5041 taacaaagag gtaacttaag attctatcag gacaaagcac tatcgtctat cacttaagat 5101 ttgaacccta ttttttatag actaattgac aacagttaga acctgattat actttgttat 5161 acttctacag taatcaagtt aaatattgaa tagggtaccg agctcgaatt catcggtagg 5221 gagatttttt caagtaacag aggttttgga ttttaaaaaa tatttccttg atttcgataa 5281 aacgaataag ttccctatga gctttgttgt taaaactgag cttgagccag aagaggttat 5341 ggacaagata aagattaact ctgaaaaaag catggaaaag gaattggtgc agaaatatct 5401 gattttgttt gaagaaattt ttacattaca gacgctcaga agtatcctgg gaatgatatc 5461 tgactctctt gatgagcttg aagagctaaa gagagtcata atcgaaaatt ttaaattaga 5521 gtatggtctt cttgattaaa taataaatca ttgtttaatg ggttagagat gttgtcaata 5581 tcatctctaa tgttttttga tttgtttttt gtcttccttt tttttacttt ctccaattct 5641 tttggggtat aatctgaagg tatacccaat cttcttatgc ccatttttac atactcttcg 5701 tttatttcta tgcctataaa tctcctgtct agagacttgg caacatctcc tgttgtaaag 5761 ctaccagaaa aagggtctaa gactacatcg cctgggttcg atgaagctag aataattctc 5821 ctcaaaaggg acgtcggttt ctgtgtgggg tggttttcat attcctccat aagaaatctt 5881 actctaggaa aatcccaaac gtttcctggt actttttgtg tgctataagg ttggggtggg 5941 ttttttctat aatcaatcaa agccctttta gatcctgttt ttgcttcaac caaaatatct 6001 ttgctgttaa atgtgtattt tttttgatct tttaccatca ttaagattgg ttcgtacatg 6061 gagccaaagt gttttttggc ttgaacacct gagctgtcat aagaccaaac taatcgactt 6121 tttatatgga agatttttct acattttatg tcaatgtatg gcatattctc agtgctgttc 6181 ataatataca tagtgccatg cttttttagc actctatggc attcatcgac gcattcgaat 6241 aaccaatgaa ggaattcacc ctcatcccat gactcaacaa gtccgtcaaa gtccttacca 6301 atattatatg gggggtctgc aaaaattaaa tcaacactct ctgctggcaa tttctttaat 6361 tcagtcaacg catcgccatg gataatttta tttttttcat ctttaaaata tctagcttca 6421 aaatctattt tcattttcta cctcttgtgt gactcatagt ctactatgtt accgatatct 6481 agatctcgag ctatcaggct agagatttta ccgccaaatc gaaccttatt agagcggttt 6541 aggctggacc ggcagttaaa attggggctt gagcggtaaa cgagtgaggg aatttcaggt 6601 aagatacttc ggatgaggag caaaaaggtg gtttatactt cctataccca aaaaggtggt 6661 ttatacttcc tataccccgg ttatccggtg gttttctgtt tgcggaataa aacagattag 6721 tgacagtttt ctcagaatgg cgtgttccgg tgacttctgt tttcgtgtgc tgcatccgga 6781 agtgtcaggc ccgccggata tgaaaaaggc ctccggagag taccgtgact ttattttcgg 6841 cacaaataca ggggtcgatg gataaatacg gcgatagttt cctgacggat gatccgtatg 6901 taccggcgga agacaagctg caaacctgtc agatggagat tgatttaatg gcggatgtgc 6961 tgagagcacc gccccgtgaa tccgcagaac tgatccgcta tgtgtttgcg gatgattggc 7021 cggaataaat aaagccgggc ttaatacaga ttaagcccgt atagggtatt attactgaat 7081 accaaacagc ttacggagga cggaatgtta cccattgaga caaccagact gccttctgat 7141 tattaatatt tttcactatt aatcagaagg aataaccatg aattttaccc ggattgacct 7201 gaatacctgg aatcgcaggg aacactttgc cctttatcgt cagcagatta aatgcggatt 7261 cagcctgacc accaaactcg atattaccgc tttgcgtacc gcactggcgg agacaggtta 7321 taagttttat ccgctgatga tttacctgat ctcccgggct gttaatcagt ttccggagtt 7381 ccggatggca ctgaaagaca atgaacttat ttactgggac cagtcagacc cggtctttac 7441 tgtctttcat aaagaaaccg aaacattctc tgcactgtcc tgccgttatt ttccggatct 7501 cagtgagttt atggcaggtt ataatgcggt aacggcagaa tatcagcatg ataccagatt 7561 gtttccgcag ggaaatttac cggagaatca cctgaatata tcatcattac cgtgggtgag 7621 ttttgacgga tttaacctga acatcaccgg aaatgatgat tattttgccc cggtttttac 7681 gatggcaaag tttcagcagg aaggtgaccg cgtattatta cctgtttctg tacaggttca 7741 tcatgcagtc tgtgatggct ttcatgcagc acggtttatt aatacacttc agctgatgtg 7801 tgataacata ctgaaataaa ttaattaatt ctgtatttaa gccaccgtat ccggcaggaa 7861 tggtggcttt tttttatatt ttaaccgtaa tctgtaattt cgtttcagac tggttcagga 7921 tcactgtacg ataatgcccc cgcagtttgg taataccctt aataaaaaag aaacagcaaa 7981 gactgacagc aataataata aagtaagcag taacaataat attaacaaca ccagatgcag 8041 ttataataat agtatttaag acaccagaaa gactgctgcg acagtcattt tgaacaacac 8101 caaaatgccg taaaggcagt agtaacaaca ccagtgaaaa catcacgata gcatagtgat 8161 atgcctgagt gtgtgtaatt aaacaataaa taaaccgcca tatataacag aagatagtat 8221 tctgaatggc atgcttttct gttcagtata aacatatcat cccggttggt ataaggatga 8281 tatataataa gttaagctga acacatattt attttggttt tattttacaa ataaagtaag 8341 acgatccgtt aagtcaaagc ggggtatatt tattataccc tgctttttta tttgtccgcc 8401 gggcgcggat aatggatcag attatgcagt gtcacaatgg ccttaccggg attggcgtaa 8461 gcgtgcggga tatccgcatg gaagcgcagg gattccccgg cagaaacggt gtgccactca 8521 tcccccagcc gcagttgtaa tgcgccttcc agtacaatga catgttctct ggttctgaaa 8581 tccatccctg tcggtgttgc ttatgcagtc tggtcgggac tcggcgtcgt cataattaca 8641 gccattgcct ggttgcttca tgggcaaaag cttgatgcgt ggggctttgt aggtatgggg 8701 ctcataattg ctgccttttt gctcgcccga tccccatcgt ggaagtcgct gcggaggccg 8761 acgccatggt gacggtgttc ggcattctga atctcaccga ggactccttc ttcgatgaga 8821 gccggcggct agaccccgcc ggcgctgtca ccgcggcgat cgaaatgctg cgagtcggat 8881 cagacgtcgt ggatgtcgga ccggccgcca gccatccgga cgcgaggcct gtatcgccgg 8941 ccgatgagat cagacgtatt gcgccgctct tagacgccct gtccgatcag atgcaccgtg 9001 tttcaatcga cagcttccaa ccggaaaccc agcgctatgc gctcaagcgc ggcgtgggct 9061 acctgaacga tatccaagga tttcctgacc ctgcgctcta tcccgatatt gctgaggcgg 9121 actgcagatg gaatgcgctt tgttacgcca gtcagaacaa tcaatgccgg accgaaccgc 9181 aaatactttg gtaataacag agggatcacc tggtacaact ttgtgtccga tcagtattcc 9241 ggctttcatg gcatcgttat accggggacg ctgagggact ctatctttgt gctggaaggc 9301 cttctggaac aggagaccgg gctgaatcca accgaaatta tgaccgatac agcaggtacc 9361 agcgaacttg tctttggcct tttctggctg ctgggatacc agttttctcc acgcctggct 9421 gatgccggtg cttcggtttt ctggcgaatg ggccatgatg ccaactatgg cgtgctgaat 9481 gatattgcca gagggcaatc agatccccga aaaatagtcc ttcagtggga cgaaatgatc 9541 cggaccgctg gctccctgaa actgggcaaa gtacaggctt cagtgctggt ccgttcattg 9601 ctgaaaagtg aacgtccttc cggactgact caggcaatca ttgaagtggg gcgcatcaac 9661 aaaacgctgt atctgcttaa ttatattgat gatgaagatt accgccggcg cattctgacc 9721 cagcttaatc ggggagaaag ccgccatgcc gttgccagag ccatctgtca cggtcaaaaa 9781 ggtgagataa gaaaacgata taccgacggt caggaagatc aactgggcgc actggggctg 9841 gtcactaacg ccgtcgtgtt atggaacact atgtatatgc aggcagccct ggatcatctc 9901 cgggcgcaag gtgaaacact gaatgatgaa gatatcgcac gcctctcccc gctttgccac 9961 ggacatatca atatgctcgg ccattattcc ttcacgctgg cagaactggt gaccaaagga 10021 catctgagac cattaaaaga ggcgtcagag gtagaaaacg ttgcttaacg tgagttttcg 10081 ttccactgag cgtcagaccc ctgtacaagg aggaaccgtt gcaccatctt cgtcttctga 10141 actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt 10201 ctgcgcgtaa tctgctgcct gtaaacgaaa aaaccgcctg gggaggcggt ttagtcgaag 10261 gttaagtcag ttggggactg cttaaccggg taactggctt tagtggagca cagataccaa 10321 atgctgtcct tccagtgtag ccgctataag gcttcaactt caagagttct gcgtatctaa 10381 atccactatc cagttaccaa tggctgctgc cagtggcgtt aagtcgtgtc ttaccgggtt 10441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 10501 cacacagccc agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct 10561 atgagaaagc gccacgcttc ccaaagggag aaaggcggac aggtatccgg taagcggcag 10621 ggtcggaaca ggagagcgca cgagggagcc accaggggga aacgcctggt atctttaagt 10681 cctgtcgggt ttcgccacct ctgacttgag cgtcggtttt tgtgatgctc gtcagggggg 10741 cggagcctat ggaaaaacgc cagctgcgtg gccttttccg ggttcttgtt ttttgctcac 10801 atgtttcttt tggtgttatc ccctgattct gtggataacc gcattaccgt ctttgagtga 10861 gctgataccg ctcgccgcag ccgaacgacc gagcggagcg agtcagtgag cgaggaagcg 10921 gaaaagcgcc tggacgagca ttttctcctt acgcatctgt gcggcatttc acacccggca 10981 tggtgcactt ttcatacaat cctcactgat gccaaatgat taagccagta tacactccgc 11041 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gctaaaaccc gctgacgcgc 11101 cctgacgggc ttgtctgctc ccggcatccg ctcacaggcc agctgtgacc gtctccggga 11161 gctgcatgtg tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag ctgcggtaaa 11221 gctcgtcagc gtggtcgtga agcgcttcca gatgtcagcc tggtcgtcgg cgtccagttc 11281 attcagtttt tccagcaggg tcagcgtctg gcttctgatg aagcgggcca tgttaagggc 11341 ggttttttcc tgtttagtca cagttctgtt tctccatgtg tggggagtct gtcggaggtt 11401 tttaatgata ccggtgaaac gcgaaaggat gctcacgata cgggtcacgg atgaggaaca 11461 tgcgcggctg ctggcacgtt gtgagggaaa acaacttgcg tcatggatgc gtaaggtctg 11521 cctggggcac cgccatcgaa aacgtcaggt cttccgacac tggcaccgcc gctgttgcgc 11581 cagttcgctt cggtcggcaa taacctgaat cagatagccc ggaagataaa cagcggacag 11641 tggtcggggc atgaccgggt tcatgtggtg gctgcactga tggccatcgg acgtgaactg 11701 agtgagctga gggatgaggt ccgtaaacag ggagaacggg atgatagtta agtttcatgc 11761 caggggaaaa ggcggtggca gtggtccggt tgattacctg ctgggccggg agcgtaaccg 11821 ggaaggggcg agagttctgc ggggtgctcc cgaagaggtg cgggaactca tcgatgccac 11881 gccgtttgcg aaaaaataca cctcgggggt tctgtctttt gcagagcaga ccctgccacc 11941 gggagagcgt gaaagggtga tggagagctt tgagcgggta ctgatgcccg gtcttgaaaa 12001 aaatcagtac agcatcctgt gggtggagca ccaggacaag gggcgtcttg agctgaattt 12061 tgtcatcccg aatatggagc tggcgagcgg aaaacggctc cagccctatt acgaccgagc 12121 ggaccgtccg cgaatcaatg cctggcagac actggtgaat caccattacg ggctgcacga 12181 cccgaacgca ccggagaaca ggcggacact tgtcaccccg aataatctgc cgaaagcgaa 12241 acaggaggcc gcagaagcga ttacgagggg actggaggct ctttaccatg ccggagcgct 12301 aaaaacgcgt caggatgtca ctgaggcgct tacagcggca ggttttgagg ttgtgaggac 12361 aaccaggagc agtatcagta tcgccgaccc ggagggtggg cgtaatctca ggctgaaagg 12421 ggcgctgtat gagcaatctt ttacaaatgg ggacagactt cgagaagaaa ctgaaagagc 12481 gtgccgtctc tacagagaac atgctgaaca gcgagttcag gaagctcgaa gcgtctgtag 12541 acagggaact gaacttaaac gggcagaaaa tcagagacgc tatcagcgca cacacagcag 12601 cggtgaaaga acaactggag acgctgaaca gtgccgtgaa cgcacagttc agcgccacag 12661 agaacgaact ggcccggcgg caggaagagc tgctctggaa gctggtcaaa gggcggatac 12721 tgtacccgtc gctgacggcg ctttgcgtca cgggcgggat ttttctggcg tcatgggggc 12781 tgattcagtg gcaggaaagc cggattgcgg gaaacatcct ggctatcagg gaccaggaag 12841 agacgctggc gaaactccgg gaaaaaacat ggggtgtgac gtatcacgag ggcaggaacg 12901 ggaaatttct ggtgctgccg tccggggtga agggggaaaa caactggacg gtggagaaga 12961 agaacgcagt aagactggtc agggagtaag tgagtatgac agagctggaa atgcacttac 13021 tgaacgcctt agagcaattg cagcaggact atacgcagcg gctgaaagac tgggagagcc 13081 ccttcacgga tttgcagcat atgttcgagc tgacacgaca ggagaacggg caactgaggg 13141 aacagtgcga cgacttgagt cagcaggtgc agagattagc cgggcaggtg gaccacttga 13201 gtcggttatt tatcacgaac agcagataag ggatgaacag gaaaggcagg cacgcctgga 13261 acgggagcgt gcgcggtcct ggcatgaaga gcaggaacgg gaagcacggg tatggcgtgg 13321 cccgtcaata gggatgtgac ggtgagttgt tccgggaggt tgccagatgg taccggaacg 13381 cgttaaaaac gcatccgggc gcgattctgg cgtgtcgttt ttgttgctgt gttttgcctc 13441 tttgccagat cgcgctcatt cctcagcctc atcccgcgat gtctgcgtat gtcatcaact 13501 gaaaagatgg aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac 13561 atggctgaat agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg 13621 ggtggtttgt tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg 13681 gtgcgtacaa ttaagggatt atggtaaatc ccttactgtc tgccctcgta tccatcgaga 13741 taaaccgcag aaatggtgtc tgccattagt ttcatttttt tcggcgaagc atatcgctgt 13801 ttgtgtattt tctgccagca aaggctgttg tgctccggag tgagtgaaaa aatttttctg 13861 agtggaattc ttttacacgt tgctgtgccg tttctgctga tactccggct gctttgtctt 13921 tcatccccat atcccggaac tgctgaaaga acgggagcat catacgtatg gtgtctgctt 13981 tctgggtgta gaagttagga tcaaatccat catctgaagc ataaacataa tgccgggtat 14041 gtcgttccag gctggtcttt ggtgtgcatc ctgtaagagc tattgttgct gttatcagag 14101 gtatcagaag cagcctggca gcggcagatc tcataaacat ctccttgcgt ttttaatctt 14161 gatactcagg gagttatatc tccgaagagt atctcttatc atacgtcggt ttactcccaa 14221 tgtgatttga tggtcaatga gtcaggaata atcccgtgaa aaaatgtgtg ccagtcctgc 14281 tttttagtgt tctttctgct tcggttgcca gtcatgcgga cagcaatgtt tttcaacaaa 14341 aggccgagaa gacaaaaaac ggcattgttg cggatgttaa tgccagacag aaaatgttca 14401 gtaacagact gaagatacaa cttcagatcg atgagttgag agcagactgg ctggcaccag 14461 agatgcccaa aaaagagagg atttgcgtaa aaaactggag tctctggagc ggcaaaagaa 14521 atccttacgc tgatgggggc gtattttccc cggtttcttc cataaccttt ttaccgttca 14581 gatatttacg ggcgaggcga atgcgtcttg cctggtatac cgccgagtaa gcctgggtgg 14641 gattcacacc attactgatt tcactgacca catgacgctg atcagccagt gtcagcgtgt 14701 aaacctgttt ctggatgagg tcacgggctt tgtcatgctc atatccggca gcaacaaggg 14761 tgtcgagaag tcgtttgtct gttttgttca gtttatggcg gggtgatttg ctcacatcgt 14821 tctccgtttt ttcatgatcc aacagtttta tatctcatcc tgatttgctc cggtgttctg 14881 tttcgacgtt ctcaatgcct gcctccccat agtgtgatcc ttattgcaaa aatgaaaatc 14941 catgctcttg acatggacaa tgctgagtag taggttttta ctgtacataa aaccagtggt 15001 tatatgtaca gtatattgtt tttaatttat tgttttaatt atcaaagagg aatttatggc 15061 taaagaacta agtggatatg gaccaactgc tggtgagtct atggggggaa caggggcgaa 15121 tctgaatcag caaggtggaa ataataatag taatagtggg gttcactggg gtggtggctc 15181 tgggcatggt aataatggtg gtcagggaaa ttcaaattca tctggttcta catcaactgt 15241 gatgaaaacc ggtgagtcat atctgactcc gtggggtgat gttgtcatta ataatgatgg 15301 ccttccggtg atgaatggta ttgttatgac agaagagaat tcaactctgg ttgataatcc 15361 atttggaggg gtctccagag ttcttaattc tttaattagt gatatgccat cactttttgc 15421 agaaagctca ggaaataaca acaataacac tgcttcagtt aatactgcac caacgaatgc 15481 tcaggtcagt gatatggata agagttcgaa ggtggtgggt aatgtaatta atgaaaaaca 15541 aaagcagaag aataaaattg ctacacagat tagtgaaaaa cagaagaaaa tcgaggagat 15601 gaaaaaagta ttcaagcatc attcttatca tggtatcact gatcttgaga gggatgttga 15661 tgagttgcaa aaaaaatcaa atcagcttga tgcagatatt agcaaactta attcttataa 15721 aaatacattg caaagtaaaa ttggtgatgt gaataaacag aaggaagctg aagaaaaggc 15781 tcgtgaaaat gcagaggtcg ctgaacatga aactcttaat gaagagaaac aggcggtggc 15841 ggaagcagag aagcgtttag ctgaggcgaa agctgaactg gcgaaggctg aaagtgatgt 15901 acagagtaag caagctactg tttccagagt tgcaggagag cttgaaaacg ctcagaagag 15961 tgttgacgtg aaggtcaccg gatttccggg atggcgtgac gttcagaaaa aactacagcg 16021 tcagcttgaa gctaagcagg ctgagtactc cgcagttgaa aatgaactta agaatgctgt 16081 tagtttcagg gatggtaagg ccgctgaggt taaagaggca gagcagaaat taaaagaagc 16141 tcaggatgct cttgaaaaga gccagataaa agatgctgtt gatactatgg ttggatttta 16201 tcaatatata accgaacaat atggagaaaa atacgcaaaa attgctcagg atttagcgga 16261 aaagtctaaa ggaaagaaaa ttcaaggtgt tgatgaagct ctggcagcat ttgaaaagta 16321 taagaatgta ctggataaga aattcagtaa ggttgatcgg gatgctattt ttaatgcgtt 16381 agagtctgtt aattatgatg agttatctaa gaatttaacg aagatatcaa agtcgcttaa 16441 aatcaccagt cgagtatctt ttctatatga tgttggttct gattttaaaa atgcaataga 16501 aactggtaac tggcgacctt tgtttgtcac gctggagaaa agtgcggtcg atgtaggtgt 16561 ggcaaaaatt gtggcattaa tgtttagttt tattgttggt gtgcctcttg gtttctgggg 16621 gattgcaatt gtcacaggta ttgtttcttc ttacataggg gatgatgagt tgagcaaact 16681 taatgagttg ttagggattt aatttctctt tagagtgtaa tatatgaaac ttggcaatcc 16741 tactgggatt gccattaata aataaaacaa tgcagataac ttgcataagt accagggttc 16801 tttttttaac tttgtatttt ctttgatata tctagatatg gttaatttag agtatggata 16861 tagcaataca tgtattgatg atataaatat aaataacaaa ggagtgttat cattgtcgtt 16921 aaatattaaa ataagaatcc atggtataag tgattcaggt aaattatgta ggtagtattt 16981 taaatgcata agtattttta cttttgttgg aaagaaatat gaaaataata atttttctaa 17041 ttacaatatt attcttgtca ggatgtcaag caaactatat tcgtgatgta cggggtctga 17101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 17161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 17221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 17281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 17341 gggcttacca tctggcccca gtgctgcaat gataccgcga gatccacgct caccggctcc 17401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 17461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 17521 agttaatagt ttgcgcaacg ttgttgccat tg //