LOCUS Exported 7308 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Forced expression of human GLIS1GR. ACCESSION . VERSION . KEYWORDS pMXs-hGLIS1GR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7308) AUTHORS Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S TITLE Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm. JOURNAL Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678. PUBMED 24759836 REFERENCE 2 (bases 1 to 7308) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7308 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1094..1510 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1440..1462 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1478..1494 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1520..1894 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1832..1848 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" LTR 4813..5282 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(5412..5429) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5583..6171) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5663..5682) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6335..7195) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6958..6977 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7196..7300) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggacctaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatcactg attttatccg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttac ctcactggaa 1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac 1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa 1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc 1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt 1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc 1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc 1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa 1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca 1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca 1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg 1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac 1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc 1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat 1921 cccagtgtgg tggtacggga attcgccctt caccatggca gaggcccgca catccctgtc 1981 tgcccactgt cggggcccgc tggccactgg cctgcaccca gacctggacc tcccgggccg 2041 aagcctcgcc acccctgcgc cttcctgcta ccttctgggc agcgaaccca gctctggcct 2101 gggcctccag cccgagaccc acctccccga gggcagcctg aagcggtgct gtgtcttggg 2161 cctacccccc acctccccag cctcctcctc accctgtgcc tcctccgacg tcacctccat 2221 catccgctcc tcccagacgt ctctggtcac ctgtgtaaat ggactccgga gcccccctct 2281 gacgggagat ctggggggcc cttccaagcg ggcccggcct ggccctgcat cgacggacag 2341 ccatgagggc agcttgcaac ttgaagcctg ccggaaggcg agcttcctga agcaggaacc 2401 cgcggatgag ttttcagagc tctttgggcc tcaccagcag ggcctgccgc ccccctatcc 2461 cctgtctcag ttgccgcctg gcccaagcct tggaggcctg gggctgggcc tggcaggcag 2521 ggtggtggcc gggcggcagg cgtgccgctg ggtggactgc tgtgcagcct atgagcagca 2581 ggaggagctg gtgcggcaca tcgagaagag ccacatcgac cagcgcaagg gcgaggactt 2641 cacctgcttc tgggctggct gcgtgcgccg ctacaagccc ttcaacgccc gctacaagct 2701 gctcatccac atgcgagtgc actcgggcga gaagcccaac aagtgcatgt ttgaaggctg 2761 cagcaaggcc ttctcacggc tggagaacct caagatccac ctgaggagcc acacgggcga 2821 gaagccgtac ctgtgccagc acccgggttg ccagaaggcc ttcagcaact ccagcgaccg 2881 cgccaagcac cagcgcaccc acctagacac gaagccgtac gcctgtcaga tccctggctg 2941 ctccaagcgc tacacagacc ccagctccct ccgcaagcac gtcaaggccc attcagccaa 3001 agagcagcag gtgcgtaaga agctgcatgc gggccctgac accgaggccg acgtcctgac 3061 cgagtgtctg gtcctgcagc agctccacac gtccacacag ctggctgcca gcgacggcaa 3121 gggtggctgt ggcctgggcc aggagctgct cccaggtgtg tatcctggct ccatcacccc 3181 ccataacgga cttgcatcgg gcctcctgcc cccagcgcac gacgtacctt ccaggcacca 3241 cccgctggat gccaccacca gttcccacca ccatctgtcc cctctgccca tggctgagag 3301 cacccgggat gggttggggc ccggcctcct ctcaccaata gtcagccccc tgaaggggct 3361 ggggccaccg ccgctgcccc catcctctca gagccattct ccggggggcc agcccttccc 3421 cacactcccc agcaagccgt cctacccacc cttccagagc cctccacccc cgcctctgcc 3481 cagcccacaa ggttaccagg gcagtttcca ctccatccag agttgcttcc cctatggcga 3541 ctgctaccgg atggctgaac cagcagccgg tggggacgga ctggtcgggg agacccacgg 3601 tttcaacccc ctgcggccca atggctacca cagcctcagc acgcccttgc ctgccacagg 3661 ctatgaggcc ctggctgagg cctcatgccc cacagcgctg ccacagcagc catctgaaga 3721 tgtggtgtcc agcggccccg aggactgtgg cttcttcccc aatggagcct ttgaccactg 3781 cctgggccac atcccctcca tctacacaga caccactagt gccatggaaa atcctggtaa 3841 caaaacaata gttcctgcaa cgttaccaca actcacccct accctggtgt cactgttgga 3901 ggttattgaa cctgaagtgt tatatgcagg atatgatagc tctgttccag actcaacttg 3961 gaggatcatg actacgctca acatgttagg agggcggcaa gtgattgcag cagtgaaatg 4021 ggcaaaggca ataccaggtt tcaggaactt acacctggat gaccaaatga ccctactgca 4081 gtactcctgg atgtttctta tggcatttgc tctggggtgg agatcatata gacaatcaag 4141 tgcaaacctg ctgtgttttg ctcctgatct gattattaat gagcagagaa tgactctacc 4201 ctgcatgtac gaccaatgta aacacatgct gtatgtttcc tctgagttac acaggcttca 4261 ggtatcttat gaagagtatc tctgtatgaa aaccttactg cttctctctt cagttcctaa 4321 ggacggtctg aagagccaag agctatttga tgaaattaga atgacctaca tcaaagagct 4381 aggaaaagcc attgtcaaga gggaaggaaa ctccagccag aactggcagc ggttttatca 4441 actgacaaaa ctcttggatt ctatgcatga agtggttgaa aatctcctta actattgctt 4501 ccaaacattt ttggataaga ccatgtccat cgagttcccc gagatgttag ctgaaatcat 4561 caccaatcag ataccaaaat attcaaatgg aaatatcaaa aaacttctgt ttcatcaaaa 4621 gtgagcggcc gccagcacag tggtcgacga taaaataaaa gattttattt agtctccaga 4681 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat 4741 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa 4801 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 4861 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa 4921 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc 4981 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct 5041 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc 5101 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact 5161 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt 5221 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt 5281 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg 5341 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6001 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 6061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa 6301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 6661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 6781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 6841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 6901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 6961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttc //