LOCUS       Exported                 654 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  Green fluorescent indicator for calcium imaging in the mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV CEPIA2mt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             43..117
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             139..213
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             253..270
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             274..306
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             310..333
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      340..349
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             361..417
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     630..651
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttctggctaa ctagagaacc cactgcttac tggcacgtga ccatgtccgt cctgacgccg
       61 ctgctgctgc ggggcttgac aggctcggcc cggcggctcc cagtgccgcg cgccaaaatt
      121 cattcactgg gggaccccat gagcgtgctc accccactcc tgctgcgggg gctgaccggc
      181 agcgctaggc ggctgccagt cccgcgggcc aagatccaca gtctcggcga tcccggatcc
      241 atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
      301 atgggtcggg atctgtacga cgatgacgat aaggatctcg ccaccatggt cgactcatca
      361 cgtcgtaagt ggaataagac aggtcacgca gtcagagcta taggtcggct gagctcactc
      421 gagaacgtct atatcatggc cgacaagcag aagaacggca tcaaggcgaa cttcaagatc
      481 cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca gaacaccccc
      541 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaaactt
      601 tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gacc
//
