LOCUS Exported 266 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter. ACCESSION . VERSION . KEYWORDS pAAV-hSyn Con/Foff hChR2(H134R)-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 266) AUTHORS Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, Deisseroth K TITLE Targeting cells with single vectors using multiple-feature Boolean logic. JOURNAL Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 2014 Jun 8. PUBMED 24908100 REFERENCE 2 (bases 1 to 266) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..266 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(95..116) /label=EGFP-N /note="EGFP, reverse primer" protein_bind complement(229..262) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." ORIGIN 1 cgagactctc gtcgaagacg aagccgaggc cggagccgtg ccagcggccg ccgtgagcaa 61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 181 cctgaagttc atctgcacca ccggcaaggt gagtacagga ggggtaccat aacttcgtat 241 aaagtatcct atacgaagtt atttgc //