LOCUS       Exported                 616 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Ratiometric (Blue to Green) fluorescent indicator for calcium 
            signaling in the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    pCIS GEM-CEPIA1er
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M
  TITLE     Imaging intraorganellar Ca(2+) at subcellular resolution using 
            CEPIA.
  JOURNAL   Nat Commun. 2014 Jun 13;5:4153. doi: 10.1038/ncomms5153.
  PUBMED    24923787
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(324..343)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agggtggcag cggtggcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
       61 tccaggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg
      121 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      181 ccgtgccctg gcccaccctc gtgaccaccc tgtcctacgg cgtgcagtgc ttcagccgct
      241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc
      301 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      361 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      421 gcaacatcct ggggcacaag ctggagtaca gcacgcgtga ccaactgact gaagagcaga
      481 tcgcanaatt taaagaggct ttctccctat ttgacaagga cggggatggg acgataacaa
      541 ccaaggatct ggggacggtg atgcggtctc tggggcagaa ccccacagaa gcagagctcc
      601 aggacatgat caatga
//
