LOCUS       Exported               12512 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGC91
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep . 180(1):103-19.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(1504..1551)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1590..1610
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1632..1652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1674..1694
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1716..1736
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1779..1807)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2555..2575
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(3217..3228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5218..5265)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5382..5402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5492..5514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9952..9974)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10074..10093
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10171..10189)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10257..10361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10362..11222
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10580..10599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11393..11981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11882..11901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12135..12152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12269..12290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12305..12335
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12343..12359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12348..12370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12367..12383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12367..12383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc
       61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa
      121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca
      181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catctagaat
      241 cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat tttgatacga
      301 cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc aagataggag
      361 cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt aataatgttc
      421 ccaacttaca aaaatgtctc cgtcgacgtt tctgatagtt actagctcga cataatcaaa
      481 atccaacgtt ttctcattca gtaaatccct ttttcgttcc aaataaagag gaagaagatg
      541 gtcatgacga cgtcgaatgt agtattgatg tgtcttggct gcttctgaaa tatttcgatg
      601 cggtatgaat ttaatctgta tatacctgtc ggtggtgtcc atccgcttgg tgtaggatac
      661 ttttcgtgct caggaacatt cggcaccact tctattggca tcaatctttc cacatacgac
      721 caatcgatag gagcttcttc gaccgttgca agacttctgg ctgtaattgc tagtagaaaa
      781 attatatgat aataaataac ttacactttt cagggagagc atgtttccat ggattttccc
      841 aaagtttctc agctttttcg actgaagttg ttgaaaaaga caacctggaa actgctcgag
      901 gggagaaaat gcggagagaa gaacccagca tcttaataaa attatataat tttgaataaa
      961 atcaaattaa aaataagatt aattgaaaca caacaataga atgcagaaaa ttactcataa
     1021 gaaataattt attttgaaag aagttgtgtc gatttacgat aaaaactaca cgcaaacgag
     1081 gctccgcgtt tacaccgttt ggcaatggag cgcgcttgct attagatttt aggccagctc
     1141 aacgaaagtt taatttattt ttcttctttt tttccatttt tttgagtaaa atatcgaatt
     1201 ttttgtatta attctgtaaa ttttataatt tgcaactatt ttggcgtatt ctgcttgttt
     1261 ttatcagcga atttttatgc atttcagtca aaagtttgag caggaaaaat gacatttagg
     1321 actttgtaaa attgtattct taaatttgct tgaaaaccta cgtgtacaat tgattttatt
     1381 gaatttttct tacgtttccc gttgtatctt attgtattta actttcatca taaaataatt
     1441 tatttccagg taaaatatcc gacgctctcg tggatccact agttctagct tcaaaagcgc
     1501 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caagatcccc
     1561 gggtaccgag ctcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggcagaaa
     1621 aaatgactgc tccaaagaag aagcgtaagg taccggcaga aaaaatgact gctccaaaga
     1681 agaagcgtaa ggtaccggca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
     1741 tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1801 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1861 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg
     1921 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg
     1981 tcactacttt ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc
     2041 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     2101 aagatgacgg gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca
     2161 tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     2221 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     2281 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg
     2341 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa
     2401 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     2461 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     2521 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     2581 ctgggattac acatggcatg gacgaactat acaaaagccc acaagctttg gaattcgcta
     2641 gccggccata caagtaatcc ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     2701 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     2761 ctttctaaaa aattaattca atccaaatct aaacatgagc ggtttttttt ctctttccgt
     2821 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct
     2881 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat
     2941 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga
     3001 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagtc tagaatccta
     3061 tgcttgcact ttgagccaat ttatccaagt ccttgtaaaa agtattcgaa aattgttaac
     3121 ggataaaatg tttattataa tatcaaaaac aatttgtcag ttgaccactt tttgatatat
     3181 ttgacagaaa ccgggatgaa ttggctcaaa gttagggcgc ccttctatta cagggtttct
     3241 gataacaaac cggttattaa ctcccaacaa gggatgattt caattcatca tgctcaaatt
     3301 gacccaaatt aagttacatg acaaattcat cgcccttttc aactcttctt ggctcatcat
     3361 ctgttattct gttcctattc tgtctgcacc ctatacccct ttgcatactc tctcgtcatt
     3421 cctttcttgg atagtgtctt cttctcccca gctctgctac ttctatgact tgcgcccgct
     3481 gcttttccgc tcgttcctct cctctctgac gtcttcttct gcttcttttc tccttccttt
     3541 ttcccttctc aatcttcctc tttttcccat ttctgtcaat cattcgaaga agaagaagaa
     3601 gaagaccctc attcatttca tttttttttc tgtcggtgtg tgctgctcgg ttaagagtga
     3661 gctctctatt ccacgtcttc ttctttttct tcttcgattc gaatcaatca ctccaccaaa
     3721 acgcattcgt ttttgggatt caccccgcgg ttcgcaacag gtttcttttt caaattatta
     3781 gcgttataaa tagaaaaatg gtggagtttc aataaaaatg ataatttaca aagtgtattt
     3841 tgattacatg tactcaaaaa ggttgaaatt tttcatacca gttttccgga aatccatctg
     3901 atatcattat cgtatttttc tttttaaaaa tgttttcaaa aaaaaacaaa atatagctgg
     3961 ttatttggca ccctctaatt accattttcc tgtcacacca cactcttttc cttcttccac
     4021 ttctttccgt tttcagccgc ttccaaccaa accgatatga aagccgagca acaacaatcg
     4081 attccacccg gctcggcgac cttcccgtcg caggtgagac tcagaaaact agagaaaccg
     4141 ctcaactaac tcttgatatc cgatttcatt cttttctctt ttctttttgt tgaacttttc
     4201 catatttcca gatgccacgg ccaccaccaa gcaccgaaca aggaatcaca acggaatcgg
     4261 agcttgcgaa gaaagctcaa atcactccga acgacgtttt agcacttccg ggaatcactc
     4321 aaggtatctt gctccctttg gatttcagtt taaaacataa atttaggatt cttatgctcc
     4381 ccatctgcga acatctataa catcgagttc accaagttcc aaatccgtga tctggacact
     4441 gagcaagtgc ttttcgagat cgccaaaccg gagaacgatc aggagaatga tgagtcgcca
     4501 caggagtcgg caagatacgt gcgttataga tttgctccaa actttttgaa actcaaaacg
     4561 gtcggagcaa ctgtggaatt caaagtagga gacatcccaa tccatcattt ccgaatgatc
     4621 gaacgtcact tcttcaaaga tcgccttctg aagtgttttg actttgaatt cggattctgt
     4681 attccgaatt cacgaaacaa ctgtgaacat atctatgagt tccctcaact ctctcaacaa
     4741 ctcagtgagt cattattcta aaaagtacaa ttcaaaagac taatctcttt ttcagtggac
     4801 gacatgatca acaatccaaa cgagactcgt tctgacagct tctatttcgt cgataacaaa
     4861 ctcgtcatgc acaacaaagc cgactactca tatgatgcat aaatatttaa tacaaaaatg
     4921 ttctggataa ttattctgtc gaatagaaaa aaaactccaa atgtgattaa attccaataa
     4981 ttcctgtcta gtttgttcct tccccttccc cttctcatgt tcaatgcatt cctaagcttt
     5041 tcagttcccc ccttgtttct atattttttc gctgtcctgt cacactcgct aaaaacacta
     5101 atcacacgga aatctgtttt caataaaaac tccaacttta actcattttc aatttcaact
     5161 gaaagatttt ttcattagag aatgtctagg catgcaagct agcttcaaaa gcgctctgaa
     5221 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat ccccgggatt
     5281 ggccaaagga cccaaaggta tgtttcgaat gatactaaca taacatagaa cattttcagg
     5341 aggacccttg gagggtaccg agctcagaaa aaatgactgc tccaaagaag aagcgtaagg
     5401 taccggtggg tgaagaccag aaacagcacc tcgaactgag ccgcgatatt gcccagcgtt
     5461 tcaacgcgct gtatggcgag atcgatcccg tcgttttaca acgtcgtgac tgggaaaacc
     5521 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5581 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg aaggtaagtt taaacagatc
     5641 catactaact aacttgttct gacataattt tcagcttgaa tggcgaatgg cgctttgcct
     5701 ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg
     5761 atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca
     5821 acgtaaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt
     5881 gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta
     5941 tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg
     6001 gccaggtaag tttaattaag ttgatactaa ctaacaaaga tctgattaat tttcaggaca
     6061 gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg
     6121 cggtgatggt gctgcgttgg agtgacggca gttatctgga agatcaggat atgtggcgga
     6181 tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt
     6241 tccatgttgc cactcgcttt aatgatgatt tctcccgcgc tgtactggag gctgaagtcc
     6301 aggtaagttt aaacaggatc ttactaacta acatgctaac actgaatttt cagatgtgcg
     6361 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
     6421 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
     6481 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
     6541 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
     6601 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
     6661 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
     6721 atgagcagac gatggtgcag gatgtaagtt taaactattc gttactaact aactttaaac
     6781 atttaaattt tcagatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc
     6841 attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg
     6901 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc
     6961 gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc
     7021 cgagtgtgat catctggtcg ctggggaatg gtaagtttaa acagttgaat actaactaac
     7081 ggagatcttt gaaattttca gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct
     7141 ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca
     7201 cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg
     7261 ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga
     7321 ttctttgcga ggtaagttta aacagaactc tactaactaa cacattagat cctaattttc
     7381 agtacgctca cgcgatgggc aacagtcttg gcggtttcgc taaatactgg caggcgtttc
     7441 gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta
     7501 aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga
     7561 acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgt
     7621 aagtttaaac aataacctac taactaacgt agataattta aattttcagg ctgacggaag
     7681 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca
     7741 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg
     7801 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga
     7861 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg
     7921 tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgca tggcagcagt
     7981 ggaggtaagt ttaaacaaga tcctactaac taactctaca ttgatgaatt ttcagactgg
     8041 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca
     8101 gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag
     8161 gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc
     8221 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
     8281 ctaacgcctg ggtcggtaag tttaaacaaa gttgtactaa ctaacgaaga tcttgataat
     8341 tttcagaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
     8401 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
     8461 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
     8521 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
     8581 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
     8641 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
     8701 agtaagttta aacttgatag tactaactaa catgtttcat ttaaattttc agtaccccgt
     8761 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac
     8821 accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg
     8881 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt
     8941 tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccaac
     9001 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt
     9061 catcagagta agtttaaact gagttctact aactaacgag taatatttaa attttcagca
     9121 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     9181 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaaacttct tcttaatttc
     9241 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     9301 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     9361 tctatcccaa aatctacaca atgttctgtg tacacttctt atgttttttt tacttctgat
     9421 aaattttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     9481 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     9541 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     9601 tatgaaatta attttcctgc ttttgctttt tgggggtttc ccctattgtt tgtcaagagt
     9661 ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag
     9721 atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa
     9781 agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca
     9841 aacataactg tttcctacta gtaaaaaata agcaaagaaa ttaggcgaac gatgagaatt
     9901 gtcctcgctt gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9961 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10021 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10081 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10141 gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta
    10201 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
    10261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10381 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10441 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10501 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10561 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10621 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10681 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10741 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    10801 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    10861 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    10921 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    10981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11221 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11521 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11761 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
    11821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    11941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12061 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12361 ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt
    12421 actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa
    12481 cgatggatac gctaacaact tggaaatgaa at
//
