LOCUS       Exported               14890 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGC200
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14890)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep . 180(1):103-19.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 14890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14890
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     443..459
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     444..460
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    475..499
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3819..3843)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3882..3929)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3968..3988
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4010..4030
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4052..4072
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4094..4114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4157..4185)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4933..4953
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(5595..5606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7596..7643)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7760..7780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7870..7892)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12330..12352)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12452..12471
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12549..12567)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12635..12739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12740..13600
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12958..12977)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13771..14359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14260..14279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14513..14530
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14647..14668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        14683..14713
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14721..14737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14726..14748
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14745..14761
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14745..14761
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagtttgt gcaacaaatt gaggaaggca ttctagagaa gaatttcggc
       61 atttcgcaat gatacaaaat gaagagaata aggaaatgac ggtgaaataa ccggtttgaa
      121 ggtaaaaatg ataaagtaga caattgaatt tttctagttc attctccaac aataaattca
      181 caacaaaaca aatctgccta gataaaaaac taaaagatat atcaagaata catctagaat
      241 cctttgctgt agaagaattg ctcaataaga acacgtggag ctcctttaat tttgatacga
      301 cctttcaact catcgacgtg actggcgatc gaatgtccaa gatgttcttc aagataggag
      361 cggagatcat tttcgcaagc ctgaaagata tttatgttga ttcaggattt aataatgttc
      421 ccaacttaca aaaatgtctc cgtcgaggtc gacggtatcg ataagcttga tatcacaagt
      481 ttgtacaaaa aagcaggctg ttcattgact gtggagcctg gaaatggagg agaaagtaaa
      541 tttgatgatt actaatactt cgctgtcttt agaggacgcg gagattgtga gacttgaaac
      601 attttataaa ttttttaatt ttactcagaa ataaaatact taatttctaa tttttttaaa
      661 gattcaacgc tgatcaaatt ctctctcatc gctggctcca acaatccgcc gcttctacga
      721 ttctccaaac tccatccaat ctcattaacc gcaaggattc ggcacgtgat gttcaacaaa
      781 tgtccgagca ctttaatctg atgaaccgac ttgcggacac tcgtctttcg gcgagattcg
      841 acaataaaat gacgatgagc gagtgtggaa gtgacttggg aactgctact attcagttag
      901 tttattattt ttaaatcttt agttctaatt taaaattctt ttcagtggtg acggatcctt
      961 cctatccatg ggtggcgaac cttttggcac tttcccacga aagaaatctg ttggaattgc
     1021 catcgaaaaa tcacgaagtg gagagttcac accgccaatc agtcgtgctt ctcctacaac
     1081 tccacctcca tcgatgctca atttgagcga agatcttact gattcaccgg tcaaaagaag
     1141 atctgccgac gattcgggaa ccttttcgct cttctctcca gcatcatcaa acggagacga
     1201 ttcgatctgt tctccaccaa tggtgtttgt cgatatgcca tctattcaac tgttcggtac
     1261 tggagcattg cttacttcag ttcaaatgac gcctcgtcac accaccgaag acgacgcatc
     1321 attgaagtcg tttgaggacg agcaggaggt ttgtttcgtc agaatctgta ataattttga
     1381 actaaactat tttaatttca gaatgcgaat cccatccatc gcatcgagac tcaggtcaac
     1441 gtctaaacca aatcttcgtc gttgctcctc cttctcattt aacacacaac ctctcaccaa
     1501 acctctcgaa ctctatctaa gatcgccact ttttttttct gtttttaaaa atccccttgt
     1561 atgtagtttt gtcattttat ttctcttgca atcaaatgca ctcttgttct catttcacac
     1621 gaatatttcg ttttgtgtgt tcgaaaatgt tggaaaaaat catctagatt ttagtattag
     1681 ttttttattt tcaacatttt tgaaaaccac caatccacca tcaattgctc tacattgtaa
     1741 atagcaagcg ttgatctgca ccatctcttc ttgttttctt tttcattttt actctttgac
     1801 ttagaaacgt gtacagtacg gggttgatca cattttcgtg ctggaaatgc gctgaatttg
     1861 tgaccaactg cgacctaatt tgcgtgtaca aatgcccatc cgccccctca actctaaaat
     1921 gcattgctgg tgttgttgtt actacgagat ccccaaattt aaacagaatg ctggaaatac
     1981 gctgttttcc catccactca ttgttgaccc gatttgttgt tgatctgaag agttttttat
     2041 tctttgcatt gacatcaatt tctgttattt tttttgtatt tatcatcttt ttatcatctt
     2101 ctttgcctac tcccgaatcc cctgtaacat catcaccaca tacttttttc taagatctga
     2161 caatcattca cattagattt tcagcagttt tcaagatttt tctagttgcc actgctgaaa
     2221 atttttcttg caaccacgtt gcaccctttt tccacatact ccccaccctc taagacctgt
     2281 aaaacacgtg tacaaaatca gagataatgt cgagtttccc agtgactagt tgagctcttt
     2341 tgattctcag atcctgaaat tcctgaaaaa gttttcaatt ctgaaagtcg agagctctcc
     2401 gagcactgct ccctatcggt gtacataccc gccttttagt cggaaaattg ttgatttgtg
     2461 aaaagagtac tgtaggagat ttttctctcg gcagttctcc ttttattacc ttccatccat
     2521 cccgaggatc gtttgcaaag cgtgtttgac atccagtctc ccaacttttt ctctgattct
     2581 cctccatttg ttgtacattt tgcttttagt ttctaacgcc taattccaac atcggccccc
     2641 agtttctttt cgtttagtag aattgacggc taaactttgg tttaccattc agaagtcacc
     2701 tttaaaatca tggttttgtc tgtcatatta ggccctctag gctcagaata ctcagaataa
     2761 gattaggaaa aaaccccaaa aaaatgaagt agtttttgta gtttgtcttc atcacggttt
     2821 ggaatagcca aacccatccc aaaccagtct tgcaaaacat ctagcttgac ttaactcccg
     2881 agaaccccac agaaagaagc ccgtgtaccc ccaaccgtcc cactcaattg ttgtacgatc
     2941 tcttgtgctg aaaatgtcga aaatgttggt gaaagctaac ttttttcgat ttttccggtt
     3001 tgtcacaaaa atccttcccc gcctatctcg ttgctggaga atacacacac acgggcctgg
     3061 ttttggtttc cttcttctcc caaatttccc aacaaaattt ttcttaatta acggaatccc
     3121 gaaatcactt taatttttat atggagttca cgctctatct cttctcgata ccattttccc
     3181 ccacgttctt cctgtccttt cttcttcata acttattttc tttgtctccc actttctctc
     3241 ttgtagtaaa ttaatatccc tttcttctgt tctgctctga aattctaatg ttatatttat
     3301 tttttataga aatgcttcac gaagttcgga aagaatgata atggtttcga atggagaata
     3361 aactgtgaat aatatattta aatgattttt tacatcacat gaaaagaaca tacaatgcat
     3421 ataagagttt gatactattg attttatctg ctacctttca cgaaacggaa attagccgtt
     3481 ttttcaaatt ttatgagtaa aaagttgtgg aatcgcaagc tacgcctcat gtgtgtacaa
     3541 aaaaataatg taaaccgatc aaaaatataa aattctagtg aaaatttgat atttcttatt
     3601 aaatcgaaaa cttcgattaa aaacatgttt tttttgtctc gtaaatccta cgtctgcaaa
     3661 caccgatgac ttagactgcg gactaggcgc caaattctac gcgcaagttg tccaagcttt
     3721 ttttctcatc atttttttct ttttctcccc ttttaaaact tttaaataac ttttgttata
     3781 aaatctaaag tttttctata tttcagctac ttttggtgac ccagctttct tgtacaaagt
     3841 ggtgatatca gatccactag ttctagcttc aaaagcgctc tgaagttcct atactttcta
     3901 gagaatagga acttcggaat aggaacttca agatccccgg gtaccgagct cagaaaaaat
     3961 gactgctcca aagaagaagc gtaaggtacc ggcagaaaaa atgactgctc caaagaagaa
     4021 gcgtaaggta ccggcagaaa aaatgactgc tccaaagaag aagcgtaagg taccggcaga
     4081 aaaaatgact gctccaaaga agaagcgtaa ggtaccggta gaaaaaatga gtaaaggaga
     4141 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
     4201 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
     4261 atttatttgc actactggaa aactacctgt tccatgggta agtttaaaca tatatatact
     4321 aactaaccct gattatttaa attttcagcc aacacttgtc actactttct gttatggtgt
     4381 tcaatgcttc tcgagatacc cagatcatat gaaacggcat gactttttca agagtgccat
     4441 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
     4501 acgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt caggtgctga
     4561 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
     4621 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
     4681 catcatggca gacaaacaaa agaatggaat caaagttgta agtttaaaca tgattttact
     4741 aactaactaa tctgatttaa attttcagaa cttcaaaatt agacacaaca ttgaagatgg
     4801 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     4861 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     4921 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     4981 cgaactatac aaaagcccac aagctttgga attcgctagc cggccataca agtaatccgg
     5041 atgatcgacg ccaacgtcgt tgaattttca aattttaaat actgaatatt tgtttttttt
     5101 cctattattt atttattctc tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat
     5161 ccaaatctaa acatgagcgg ttttttttct ctttccgtct cccaattcgt attccgctcc
     5221 tctcatctga acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt
     5281 ggggggaatt ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac
     5341 acaatatgca acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa
     5401 atatatagag gaggaaggaa aactagtcta gaatcctatg cttgcacttt gagccaattt
     5461 atccaagtcc ttgtaaaaag tattcgaaaa ttgttaacgg ataaaatgtt tattataata
     5521 tcaaaaacaa tttgtcagtt gaccactttt tgatatattt gacagaaacc gggatgaatt
     5581 ggctcaaagt tagggcgccc ttctattaca gggtttctga taacaaaccg gttattaact
     5641 cccaacaagg gatgatttca attcatcatg ctcaaattga cccaaattaa gttacatgac
     5701 aaattcatcg cccttttcaa ctcttcttgg ctcatcatct gttattctgt tcctattctg
     5761 tctgcaccct ataccccttt gcatactctc tcgtcattcc tttcttggat agtgtcttct
     5821 tctccccagc tctgctactt ctatgacttg cgcccgctgc ttttccgctc gttcctctcc
     5881 tctctgacgt cttcttctgc ttcttttctc cttccttttt cccttctcaa tcttcctctt
     5941 tttcccattt ctgtcaatca ttcgaagaag aagaagaaga agaccctcat tcatttcatt
     6001 tttttttctg tcggtgtgtg ctgctcggtt aagagtgagc tctctattcc acgtcttctt
     6061 ctttttcttc ttcgattcga atcaatcact ccaccaaaac gcattcgttt ttgggattca
     6121 ccccgcggtt cgcaacaggt ttctttttca aattattagc gttataaata gaaaaatggt
     6181 ggagtttcaa taaaaatgat aatttacaaa gtgtattttg attacatgta ctcaaaaagg
     6241 ttgaaatttt tcataccagt tttccggaaa tccatctgat atcattatcg tatttttctt
     6301 tttaaaaatg ttttcaaaaa aaaacaaaat atagctggtt atttggcacc ctctaattac
     6361 cattttcctg tcacaccaca ctcttttcct tcttccactt ctttccgttt tcagccgctt
     6421 ccaaccaaac cgatatgaaa gccgagcaac aacaatcgat tccacccggc tcggcgacct
     6481 tcccgtcgca ggtgagactc agaaaactag agaaaccgct caactaactc ttgatatccg
     6541 atttcattct tttctctttt ctttttgttg aacttttcca tatttccaga tgccacggcc
     6601 accaccaagc accgaacaag gaatcacaac ggaatcggag cttgcgaaga aagctcaaat
     6661 cactccgaac gacgttttag cacttccggg aatcactcaa ggtatcttgc tccctttgga
     6721 tttcagttta aaacataaat ttaggattct tatgctcccc atctgcgaac atctataaca
     6781 tcgagttcac caagttccaa atccgtgatc tggacactga gcaagtgctt ttcgagatcg
     6841 ccaaaccgga gaacgatcag gagaatgatg agtcgccaca ggagtcggca agatacgtgc
     6901 gttatagatt tgctccaaac tttttgaaac tcaaaacggt cggagcaact gtggaattca
     6961 aagtaggaga catcccaatc catcatttcc gaatgatcga acgtcacttc ttcaaagatc
     7021 gccttctgaa gtgttttgac tttgaattcg gattctgtat tccgaattca cgaaacaact
     7081 gtgaacatat ctatgagttc cctcaactct ctcaacaact cagtgagtca ttattctaaa
     7141 aagtacaatt caaaagacta atctcttttt cagtggacga catgatcaac aatccaaacg
     7201 agactcgttc tgacagcttc tatttcgtcg ataacaaact cgtcatgcac aacaaagccg
     7261 actactcata tgatgcataa atatttaata caaaaatgtt ctggataatt attctgtcga
     7321 atagaaaaaa aactccaaat gtgattaaat tccaataatt cctgtctagt ttgttccttc
     7381 cccttcccct tctcatgttc aatgcattcc taagcttttc agttcccccc ttgtttctat
     7441 attttttcgc tgtcctgtca cactcgctaa aaacactaat cacacggaaa tctgttttca
     7501 ataaaaactc caactttaac tcattttcaa tttcaactga aagatttttt cattagagaa
     7561 tgtctaggca tgcaagctag cttcaaaagc gctctgaagt tcctatactt tctagagaat
     7621 aggaacttcg gaataggaac ttcaagatcc ccgggattgg ccaaaggacc caaaggtatg
     7681 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttgga gggtaccgag
     7741 ctcagaaaaa atgactgctc caaagaagaa gcgtaaggta ccggtgggtg aagaccagaa
     7801 acagcacctc gaactgagcc gcgatattgc ccagcgtttc aacgcgctgt atggcgagat
     7861 cgatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7921 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7981 cccttcccaa cagttgcgaa ggtaagttta aacagatcca tactaactaa cttgttctga
     8041 cataattttc agcttgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
     8101 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
     8161 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt
     8221 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
     8281 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc
     8341 gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggtaagtt taattaagtt
     8401 gatactaact aacaaagatc tgattaattt tcaggacagt cgtttgccgt ctgaatttga
     8461 cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag
     8521 tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
     8581 ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
     8641 tgatgatttc tcccgcgctg tactggaggc tgaagtccag gtaagtttaa acaggatctt
     8701 actaactaac atgctaacac tgaattttca gatgtgcggc gagttgcgtg actacctacg
     8761 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
     8821 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt
     8881 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
     8941 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
     9001 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa
     9061 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
     9121 tgtaagttta aactattcgt tactaactaa ctttaaacat ttaaattttc agatcctgct
     9181 gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg
     9241 gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca
     9301 cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga
     9361 acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct
     9421 ggggaatggt aagtttaaac agttgaatac taactaacgg agatctttga aattttcaga
     9481 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
     9541 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
     9601 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
     9661 aaaatggctt tcgctacctg gagagacgcg cccgctgatt ctttgcgagg taagtttaaa
     9721 cagaactcta ctaactaaca cattagatcc taattttcag tacgctcacg cgatgggcaa
     9781 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     9841 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     9901 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     9961 cggtctggtc tttgccgacc gcacgccgca tccagcgtaa gtttaaacaa taacctacta
    10021 actaacgtag ataatttaaa ttttcaggct gacggaagca aaacaccagc agcagttttt
    10081 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag
    10141 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga
    10201 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca
    10261 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc
    10321 atggtcagaa gccgggcaca tcagcgcatg gcagcagtgg aggtaagttt aaacaagatc
    10381 ctactaacta actctacatt gatgaatttt cagactggcg gaaaacctca gtgtgacgct
    10441 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
    10501 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
    10561 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
    10621 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcggtaagtt
    10681 taaacaaagt tgtactaact aacgaagatc ttgataattt tcagaacgct ggaaggcggc
    10741 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
    10801 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
    10861 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
    10921 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
    10981 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
    11041 ctgttttgac cgctgggatc tgccattgtc agacatgtag taagtttaaa cttgatagta
    11101 ctaactaaca tgtttcattt aaattttcag taccccgtac gtcttcccga gcgaaaacgg
    11161 tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
    11221 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
    11281 gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
    11341 cgactcctgg agcccgtcag tatcggcgga attccaactg agcgccggtc gctaccatta
    11401 ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga
    11461 gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact
    11521 tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc
    11581 tatttttgtt attatcaaaa aaacttcttc ttaatttctt tgttttttag cttcttttaa
    11641 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
    11701 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
    11761 gttctgtgta cacttcttat gtttttttta cttctgataa attttttttg aaacatcata
    11821 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
    11881 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
    11941 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
    12001 ttgctttttg ggggtttccc ctattgtttg tcaagagttt cgaggacggc gtttttcttg
    12061 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
    12121 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
    12181 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
    12241 aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg gccctttcgt
    12301 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    12361 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    12421 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
    12481 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg
    12541 ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    12601 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    12661 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    12721 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    12781 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    12841 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    12901 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    12961 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    13021 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    13081 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    13141 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    13201 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    13261 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    13321 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    13381 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    13441 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    13501 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    13561 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    13621 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    13681 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    13741 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    13801 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    13861 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    13921 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    13981 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    14041 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    14101 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    14161 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    14221 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    14281 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    14341 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    14401 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    14461 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    14521 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    14581 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    14641 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    14701 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    14761 ccatgattac gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta
    14821 aattttcaga gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg
    14881 gaaatgaaat
//
