LOCUS       Exported                1050 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  Expresses LexA-ER-B112 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ottoz DS, Rudolf F, Stelling J
  TITLE     Inducible, tightly regulated and growth condition-independent 
            transcription factor in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2014 Jul 17. pii: gku616.
  PUBMED    25034689
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      93..342
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     308..326
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ggccgaagct gggtaccaag cagacgctac taaggaaaac aacaacggaa gcgatgatga
       61 gagcgacgat gagagtgtaa actgcgaacc ttgcaaatta aagccttcga gcgtcccaaa
      121 accttctcaa gcaaggtttt cagtataatg ttacatgcgt acacgcgttt gtacagaaaa
      181 aaaagaaaaa tttgaaatat aaataacgtt cttaatacta acataactat taaaaaaaat
      241 aaatagggac ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg
      301 gaggagggcg tgaatgtaag cgtgacataa ctaattacat gatatagaca aaggaaaagg
      361 ggcctgtctc gaggataagc tttaaacgcg tttaaagctt gaaacacaaa tcagtaccac
      421 cagaatgtct ttcagctcta aattcaccag aggatcttct ttcagcaact ggatcgaaat
      481 catcagcaga tggaatagct tcttcgaatt tttccaaacc agaatcgaaa ttaactttag
      541 catctttaac agtcaaagtt tcccaaatca aatcagattg ttgttccaaa acaccaatag
      601 cttcacccaa agaagcaaca gcagtatcca atttattaac agaacctgga gcatcgaaaa
      661 ccaattcagc aataccatct tccaaccaat ccaaatacaa agtatcacct ttatacaaca
      721 tatcagtttc ttggattctc aaagtaatac ctggaaattc gcccgcggtg ctgttatcgt
      781 tgcccggcag atcctgcatg atatcgactg tggcagggaa accctctgcc tcccccgtga
      841 tgtaatactt ttgcaaggaa tgcgatgaag tagagcccgc agtggccaag tggctttggt
      901 ccgtctcctc cacggatgcc cctccacggc tagtgggcgc atgtaggcgg tgggcgtcca
      961 gcatctccag cagcaggtca tagaggggca ccacgttctt gcacttcatg ctgtacagat
     1021 gctccatgcc tttgttactc atgtgcctga
//
