LOCUS Exported 794 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION Light insensitive CRY2 mutant. ACCESSION . VERSION . KEYWORDS pCMV-CRY2(D387A)-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 794) AUTHORS Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD TITLE Reversible protein inactivation by optogenetic trapping in cells. JOURNAL Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4. PUBMED 24793453 REFERENCE 2 (bases 1 to 794) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..794 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(16..723) /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(291..310) /label=mCherry-F /note="mCherry, forward primer" primer_bind 560..578 /label=mCherry-R /note="mCherry, reverse primer" regulatory 720..729 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 agatctgagt ccggacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc 61 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat 121 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac 181 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag 241 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg 301 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc 361 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat 421 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc 481 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc 541 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg 601 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg 661 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac 721 catggtggcg accggtgggc tgccgctgcc ggcagcaccg atcataatct gcgcctccga 781 gtgcgagaga tcgc //