LOCUS       Exported                 944 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  An empty gRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K
  TITLE     Genome-wide recessive genetic screening in mammalian cells with a 
            lentiviral CRISPR-guide RNA library.
  JOURNAL   Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 
            2013 Dec 23.
  PUBMED    24535568
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     128..147
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        222..297
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        320..819
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(355..378)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     752..771
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(859..878)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
       61 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      121 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      181 tatcttgtgg aaaggacgaa acaccgggtc ttcgagaaga cgttttagag ctagaaatag
      241 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
      301 ttttggatcc aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
      361 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
      421 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
      481 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
      541 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
      601 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
      661 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
      721 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
      781 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc
      841 gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
      901 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccga
//
