LOCUS Exported 1450 bp ds-DNA linear SYN 27-MAY-2021 DEFINITION pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography. ACCESSION . VERSION . KEYWORDS Traptavidin-E6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1450) AUTHORS Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, Dushek O, van der Merwe PA, Howarth M TITLE SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. JOURNAL J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21. PUBMED 25111182 REFERENCE 2 (bases 1 to 1450) AUTHORS . TITLE Direct Submission JOURNAL Exported May 27, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1450 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 18..40 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 477..494 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(547..565) /label=T7 Term /note="T7 terminator, reverse primer" terminator 561..608 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 645..1098 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(732..751) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 942..963 /label=F1ori-F /note="F1 origin, forward primer" gap 1325..1337 /estimated_length=13 gap 1435..1449 /estimated_length=15 ORIGIN 1 ttccnnnnna nannnntttt gtttaacttt aagaaggaga tatacatatg gctgaagctg 61 gtatcaccgg cacctggtac aaccagctgg gatccacctt catcgttacc gctggtgctg 121 acggtgctct gaccggtacc tacgaatccg ctgttggtaa cgctgaaggc gattacgttc 181 tgaccggtcg ttacgactcc gctccggcta ccgacggttc cggaaccgct ctgggttgga 241 ccgttgcttg gaaaaacaac taccgtaacg ctcactccgc taccacctgg tctggccagt 301 acgttggtgg tgctgaagct cgtatcaaca cccagtggtt gttgacctcc ggcaccaccg 361 aagctaacgc gtggaaatcc accctggttg gtcacgacac cttcaccaaa gttaaaccgt 421 ccgctgcttc cgaagaggaa gaggaagagt aataaaagct tgcggccgca ctcgagcacc 481 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg 541 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg 601 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg 661 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 721 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 781 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 841 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 901 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 961 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataanggn ntttgccgat 1021 ttcggcctat tggttaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 1081 tattaacgct tacaatttag nggcactttt cggggaaatg tgcgcgaacc ctatttgttt 1141 atttttctaa atacattcaa tatgtatccg ctcatganan anancctgat aatgcttcan 1201 taatattgaa aaggaagagt atgagtattc acatttccgn gtcgcctnat tccntttttt 1261 gngnnatttg cnncnnnttt nnnnnnccnn aaannctggt gaagtaaana tgctganatc 1321 antgnnnnnn nnnnnnncnt cnantnnnnn nnancgnaan attcntgann nntnnncccn 1381 nanannnncn nnnnngnnnn nnttnannnc tgcnnnnnng gnnngnntnt tntcnnnnnn 1441 nnnnnnnnng //