LOCUS Exported 11958 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5. ACCESSION . VERSION . KEYWORDS pTK333 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11958) AUTHORS Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, Weiss R TITLE Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. JOURNAL Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301. PUBMED 26237515 REFERENCE 2 (bases 1 to 11958) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11958 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 7612..7621 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7621..8007 /codon_start=1 /product="bacteriophage MS2 coat protein" /label=MS2-N55K /note="binds with high affinity to a specific stem-loop structure in the viral RNA (Lim et al., 1994)" /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI VKAMQGLLKDGNPIPSAIAANSGIY" CDS 8884..8940 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 8941..9660 /codon_start=1 /product="enhanced blue variant of GFP (Ai et al., 2007)" /label=EBFP2 /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL EFRTAAGITLGMDELYK" primer_bind complement(8986..9007) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(10043..10061) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10129..10233 /gene="bla" /label=AmpR promoter CDS 10234..11094 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10452..10471) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11265..11853 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11754..11773 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 11941..1 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 11941..11958 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa 61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc 121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta 181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag 241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc 301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta 361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc 421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg 481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg 541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca 601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg 661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag 721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt 781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc 841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg 901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa 961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca 1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg 1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg 1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc 1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg 1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct 1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct 1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt 1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac 1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg 1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca 1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag 1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga 1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag 1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg 1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag 1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc 1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca 2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt 2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct 2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa 2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca 2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg 2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg 2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag 2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc 2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa 2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta 2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga 2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga 2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga 2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca 2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg 2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca 3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa 3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt 3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc 3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct 3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac 3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca 3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta 3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa 3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct 3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca 3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg 3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt 3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc 3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc 3841 tgaattgcct taactcagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca 3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac 3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc 4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta 4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact 4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct 4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca 4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc 4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag 4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt 4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca 4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg 4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg 4621 atgagttagt atggatccat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta 4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca 4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct 4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt 4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg 4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc 4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc 5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg 5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag 5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag 5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt 5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc 5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg 5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct 5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg 5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt 5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg 5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc 5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg 5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc 5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc 5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg 5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg 6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata 6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac 6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt 6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc 6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata 6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc 6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg 6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg 6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta 6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc 6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag 6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg 6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc 6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag 6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc 6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac 6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg 7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca 7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca 7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa 7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg 7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag 7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg 7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta 7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata 7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca 7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tgccaccatg 7621 gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactggcga cgtgactgtc 7681 gccccaagca acttcgctaa cggggtcgct gaatggatca gctctaactc gcgttcacag 7741 gcttacaaag taacctgtag cgttcgtcag tcctctgcgc agaagcgcaa atacaccatc 7801 aaagtcgagg tgcctaaagt ggcaacccag actgttggtg gtgtagagct tcctgtagcc 7861 gcatggcgtt cgtacttaaa tatggaacta accattccaa ttttcgccac gaattccgac 7921 tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctcg 7981 gccatcgcag caaactccgg catctactcg atcgccatgc cagcggcaac tgtagatcat 8041 agccaaagaa tttgtgaagt ttgggcttgc aacttggatg aagagatgaa gaaaattcgt 8101 caagttatcc gaaaatataa ttacgttgct atggacaccg agtttccagg tgtggttgca 8161 agacccattg gagaattcag gagcaatgct gactatcaat accaactatt gcggtgtaat 8221 gtagacttgt taaagataat tcagctagga ctgacattta tgaatgagca aggagaatac 8281 cctccaggaa cttcaacttg gcagtttaat tttaaattta atttgacgga ggacatgtat 8341 gcccaggact ctatagagct actaacaaca tctggtatcc agtttaaaaa acatgaggag 8401 gaaggaattg aaacccagta ctttgcagaa cttcttatga cttctggagt ggtcctctgt 8461 gaaggggtca aatggttgtc atttcatagc ggttacgact ttggctactt aatcaaaatc 8521 ctaaccaact ctaacttgcc tgaagaagaa cttgacttct ttgagatcct tcgattgttt 8581 tttcctgtca tttatgatgt gaagtacctc atgaagagct gcaaaaatct caaaggtgga 8641 ttacaggagg tggcagaaca gttagagctg gaacggatag gaccacaaca tcaggcagga 8701 tctgattcat tgctcacagg aatggccttt ttcaaaatga gagaaatgtt ctttgaagat 8761 catattgatg atgccaaata ttgtggtcat ttgtatggcc ttggttctgg ttcatcctat 8821 gtacagaatg gcacagggaa tgcatatgaa gaggaagcca acaagcagtc agttggatct 8881 ggcgccacca acttctctct gctgaagcag gccggcgacg tggaggagaa cccaggccca 8941 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 9001 ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg gcgagggcga tgccaccaac 9061 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 9121 ctcgtgacca ccctgagcca cggcgtgcag tgcttcgccc gctaccccga ccacatgaag 9181 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 9241 ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg cgacacccta 9301 gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg acggcaacat cctggggcac 9361 aagctggagt acaacttcaa cagccacaac atctatatca tggccgtcaa gcagaagaac 9421 ggcatcaagg tgaacttcaa gatccgccac aacgtggagg acggcagcgt gcagctcgcc 9481 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacagccac 9541 tacctgagca cccagtccgt gctgagcaaa gaccccaacg agaagcgcga tcacatggtc 9601 ctgctggagt tccgcaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 9661 actagtaaaa gcactctgat ttgacaatta aagcactctg atttgacaat taaagcactc 9721 tgatttgaca attaaagcac tctgatttga caattaaagc ggccgcgact ctagatcata 9781 ctcgaggaag cgcagtacat aatgctgcgc agtgttgcca cataaccact atattaacca 9841 tttatctagc ggacgccaaa aactcaatgt atttctgagg aagcgtggtg cataatgcca 9901 cgcagcgtct gcataacttt tattatttct tttattaatc aacaaaattt tgtttttaac 9961 atttcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aggggaattc gagctcggga 10021 attaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 10081 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 10141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 10201 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 10261 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 10321 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 10381 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 10441 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 10501 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 10561 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 10621 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 10681 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 10741 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 10801 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 10861 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 10921 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 10981 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 11041 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 11101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 11161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 11221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 11281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 11341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 11401 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 11461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 11521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 11581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 11641 tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 11701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 11761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 11821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcgag ctcgtatgga 11881 catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg 11941 atttaggtga cactatag //