LOCUS       Exported                 806 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, 
            EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    pLKO5.sgRNA.EFS.tRFP657
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(48..749)
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELITENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKV
                     VEGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 caaattttgt aatccagagg ttgattgtcg acttaacgcg tggtacctta attaagcttg
       61 tgccccagtt tgctagggag gtcgcagtat ctggccacag ccacctcgtg ctgctcgacg
      121 taggtctctt tgtcggcctc cttgattctt tccagtctgt agtccacata gtagacgccg
      181 ggcatcttga ggttcttagc gggtttcttg gatctgtatg tggtcttgaa gttgcagatc
      241 aggtggcccc cgcccacgag cttcagggcc agcgcggttc tgccttccag gccgccgtca
      301 gcggggtaca gcatctcggt gtgggcctcc cagccgagtg ttttcttctg catcacaggg
      361 ccgttggatg ggaagttcac ccctctgatc ttgacgttgt agatgaggca gccgtcctgg
      421 aggctggtgt cctgggtagc ggtcagcacg cccccgtctt cgtatgtggt gactctctcc
      481 catgtgaagc cctcagggaa ggactgcttc cagaagtcgg ggatgccctg ggtgtggttg
      541 atgaaggtgt gactgccgta catgaagctg gtagccagga tgtcgaaggc gaaggggaga
      601 gggccgccct cgaccacctt gattctctgg gtctgggtgc cctcgtaggg cttgccttcg
      661 ccctcggatg tgcacttgaa gtggtggttg ttcacggtgc cctccatgta cagcttcatg
      721 tgcatgttct cggtgatcag ctcgctcatg gtggcagatc ccgcgtcacg acacctgtgt
      781 tctggcggca aacccgttgc gaaaaa
//
