LOCUS Exported 3411 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pGC193 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3411) AUTHORS Voutev R, Hubbard EJ TITLE A ""FLP-Out"" system for controlled gene expression in Caenorhabditis elegans. JOURNAL Genetics. 2008 Sep . 180(1):103-19. PUBMED 18723890 REFERENCE 2 (bases 1 to 3411) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3411 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 593..688 /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1520..1615) /gene="mutant version of attL" /label=attL3 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1652..1671) /label=T7 /note="T7 promoter, forward primer" promoter complement(1653..1671) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1676..1692) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1676..1692) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1794..1813) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1805..2614 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1872..1891) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2761..3349 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3250..3269 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg 601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 661 ataatgccaa ctttgtatag aaaagttgat ggcgaagaaa ccgagagtta ccaagctgaa 721 tacggatcga agatcgagag caaacgagta cgttctcaaa ttaattacga taaaattaac 781 attttcttga atttttttag tagttaatac gactcctagc aataaaattg aatataatat 841 gaaaattagt tagaaaaaaa tataccggtc agaactcaag cttcatgaag aactgtttct 901 agatagaatg ccccacattt ttcaatatct cagaaatcac atgcttcatc atcaaaggtt 961 tattaaacat tgtaaacttt cagacgagaa cgacagaaag tttccgagat gaacggaatg 1021 tttgatgttc tacttaatct tcttccgccg tcacatttta aaacacgact ctccagagtt 1081 caaatcttga ggtaagttcc ccaataagat gagatctctt ttagaaaatt ttcaaaactc 1141 tacggaagtt tacttgtttg ttttcttaat tcaaagattt tcattaaaaa aataaccctc 1201 gcatttcaga gaagccacct cttacataat tcgtcttcac aactttctta ttgaaagttc 1261 aaatagtgac atagacgcaa taactgtatt tccgcacatc ttcaacggag aacgcaagtc 1321 aaataaagat gcaattcgtc gtcccatgaa gttgaagcag ggtggcggtg ttgctgcttt 1381 catctcacgc cacgaacttc ccccacttca acttccaaat cccgttatcc caattttgaa 1441 gcccacttca gttccagttt ggccccaaac caatgtttat attgcttatt tttaaattat 1501 tgagtttgta tttgagttgc aactttatta tacaaagttg gcattataaa aaagcattgc 1561 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttggagc 1621 tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt acatggtcat 1681 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga 1741 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 1801 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc 1861 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta 1921 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt 1981 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct 2041 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat 2101 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt 2161 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt 2221 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt 2281 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga 2341 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact 2401 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg 2461 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc 2521 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt 2581 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca 2641 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa 2701 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 2761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 2821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 2881 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 2941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 3001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 3061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 3121 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg 3181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 3241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 3301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 3361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t //