LOCUS       Exported               11111 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGC81
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep . 180(1):103-19.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 11111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7998..8017)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7999..8017)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8024..8041)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8024..8040)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8033..8055)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8178..8480
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8359..8378
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8829..9623
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8883..8902)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9493..9512
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      9824..9833
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9830..10204
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      10342..10930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10831..10850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11084..11101
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgacg cgttagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttcatc acttgaatgt tcacagattc
      361 cgatcaatat ccatctcacc agatcctctg tggtgtgctc attttctatc cattttccat
      421 taaaaacacg tgatgttcgt ggtcatccac agccttgtgc tcctccaagt gctccaagaa
      481 atggccagtt gtcatttcat ttttctcatc gtctccttga tccaagtact attttcacgt
      541 tttataagaa aaaccaaata ctctctactc ttgttccatt aacttggctg gcctgcagtc
      601 gatcggtcac tttcttccag cataacattt ctggatgcaa agtgggaatt ctatgaattt
      661 taagtctgaa aattaaatac ctaatttctc aaaatttagt tctgtgtaac ggaaatacag
      721 actaattgtt taatcttttc tcccactatc tctgctccgt tcaacattat tctcttcagt
      781 ataatttgca ttccgttcca ttaaacattt acaaaccatt cttctcactt tatcagtttt
      841 aattttgttc tcgaggaata aattataaaa ataaatcttc tcagaaatga caataaaata
      901 gtcagacaac tgctctcatg ctaataataa tgtcaagaat gtcatcccca aagtcataaa
      961 tccttatttt ttcgtatcgg gtcaccagat tgttgatatc agcccgcctc ctgttacact
     1021 ttgctcttct accaaaccgc ccatctagcc ccatctcaaa tgctcacaat cttggggctc
     1081 aaaccatcaa cacattccta gtttattttt gttttatttt ccattgttta gctcatattt
     1141 tctgatcatc tgaaatcttc cctacatccc cgtagtatcg tttgagtttt aatttgcgat
     1201 cctcaaattt cacaatatat ttatttttct atcataacat ggaatctttt aagacatctt
     1261 ataatttttc agaatgaagt atttcatcct gacacttgcc gttctcggct caactcttgc
     1321 atattcaaag cagaatatgc ttgacacaaa ccgtgttcag gctgaagtaa ttgagaactc
     1381 atcttgtgaa gaatgtgaga atgttatcaa aagaatttct gaagctgcta aggatccaaa
     1441 gaaaattgaa gaattgaaga ttgttctcgg aatgatgtgc agagagactt cttatgctga
     1501 tgaatgtcgt cttttcgttt cacaattgga caagttcatt gacaaacttc agccattctt
     1561 ggttagttaa atctttgttt ctgaaaatat atgcttatat ctttgatttt ttagaagaat
     1621 ccacacgcag tctgcactcg tcttcatatc tgctcaaaca agaaaatcga ctcattccgc
     1681 cgcattttgc tcgagttcgc caagcgcgct caaaaaactt ttgacgctgt gagtagaaat
     1741 tattaaattt aaaattataa cgatgaaagt taagccagcc atcgtctgtg acgaatgcca
     1801 atttgtcgtc aaggagttga agactgttgt tgaggacaag aagagccaag ctgaagcccg
     1861 tgacttcctt cgtgagaacg tgtgcaagag cctcggacag tacagaggat tcgtaagttc
     1921 ctaaaaatta caagcttcga tttttatagt aaattttcag tgcgatttgg tcgttgacga
     1981 gtatcttcca caattcatcc aagagcttga cgccattctc caagatccac accaggttcg
     2041 ttatccattc aattataaaa aacagaaatt ccttggattt gtctaatttg taaagtagaa
     2101 gatttcaata ttttcaggtg tgcgttgaca tcaaggcctg caactctgct tctgctcaag
     2161 gactcaaggc tcgcaaatat gtgggtatcc tcggatattt ccaacgtcac ttcctataaa
     2221 tatgtttccc attaagcttt attctccttt tttgcataaa cctattttat tgtgaattgt
     2281 ctatttttcc catgttccct tccataaatc gcttgttttg tctataaatc cctttgttgt
     2341 gacgttttga tttcagattt ttaggttaag ttctttaatt tattcaattg aattgttata
     2401 taagttcagg tagaagcttc caatgaagaa aaggaaaagg ttgagaatga ggtcgatgca
     2461 ttctggaatg gagccgcaat gaaaaacaac aagggtcagg ttagtgaaat gatagggtac
     2521 tgtatttctt gttgtaccat agattgattc taaattagta tttgaaacaa ctatacaaaa
     2581 acatgtttac ctattttcgc gcatttcaac tcaacttttt tattcgcagc tgtcaagtat
     2641 gccacctgct attcgaggta taatgggttt ttttaaacta ctaatgaaca accgttctac
     2701 ttcattcaac tcaaatacaa actcaataat ttaaaaataa gcaatataaa cattggtttg
     2761 gggccaaact ggaactgaag tgggcttcaa aattgggata acgggatttg gaagttgaag
     2821 tgggggaagt tcgtggcgtg agatgaaagc agcaacaccg ccaccctgct tcaacttcat
     2881 gggacgacga attgcatctt tatttgactt gcgttctccg ttgaagatgt gcggaaatac
     2941 agttattgcg tctatgtcac tatttgaact ttcaataaga aagttgtgaa gacgaattat
     3001 gtaagaggtg gcttctctga aatgcgaggg ttattttttt aatgaaaatc tttgaattaa
     3061 gaaaacaaac aagtaaactt ccgtagagtt ttgaaaattt tctaaaagag atctcatctt
     3121 attggggaac ttacctcaag atttgaactc tggagagtcg tgttttaaaa tgtgacggcg
     3181 gaagaagatt aagtagaaca tcaaacattc cgttcatctc ggaaactctc tgtcgttctc
     3241 gtctgaaagt ttacaatgtt taataaacct ttgatgatga agcatgtgat ttctgagata
     3301 ttgaaaaatg tggggcattc tatctagaaa cagttcttca tgaagcttga gttctgaccg
     3361 gtatattttt ttctaactaa ttttcatatt atattcaatt ttattgctag gagtcgtatt
     3421 aactactaaa aaaattcaag aaaatgttaa ttttatcgta attaatttga gaacgtactc
     3481 gtttgctctc gatcttcgat ccgtattcag cttggtaact ctcggtttct tcgccatttt
     3541 aataaaattg tgtaagatga cgctataaaa aatgacgagt tgtgagccaa gtaggagttg
     3601 agtaatgaaa agaaaatgac agatgtaact gaccctttac catctaaaaa aggaatcgtg
     3661 tgccgtccca acagccacct gcttcacaca tgttaccctt ctactgacat tgtcgagaca
     3721 aagtcggtaa ataattggaa catgatgata ttgctcataa gtttatagat ggaaagagat
     3781 ttcgttgtta ttttgcaaga aaaagggaat ttgatgtggt ttcttatttt tcaaaatttg
     3841 aatagaagca atacactccc atttttcaga ctctcgccat gtcctgtttc gaatgcaagt
     3901 ttgttgttga agacatgcaa acagacatga tcactaacag agtaagttta ttgcaaaaac
     3961 aattaaaata gtttcgtaac ataaaaacaa caatccaact gtgtatttgt ttatttcaat
     4021 cacctacacc aaaacaaatt tttaaatgat tcacctctag tgatttcaga agaaactttc
     4081 cgatgatgtc cgtgatttcg catgctacaa gattgtcaca gccaacatga ctgattcatg
     4141 tattgatttc ttggatttat atctcccaac tgttattcag atgacagttg aacaggcaac
     4201 tccacaagga gtttgtctag ctaacaagtg ttgtccagca agttcaggtt tgctgtaata
     4261 atgaggtgtc caataatcga ccgactattt ttcagttgcc ccacttcgtg atttcacatt
     4321 ccaagagatt caagctgaaa aatgtccgaa aatgatggaa ttggaaacat atttgagctc
     4381 aaaccttgtt ggaagtgcca tggagaaatt cttcgaaaac tcactgactg agactttctg
     4441 ctctggatta ttatcattct tccaaccaag tgtaagtttt tattagtttt ttttaatgtt
     4501 ctaaagctta aatctcaatt ttcagtgtca acaaatcatg tcatctgttg ctccaagatt
     4561 tgtcagtttg accgcagtcc ttgcaagcca aaacaaattc agtcaggctg tgagtttggg
     4621 tattcgaatg ctcgatttta attaaaatat tccagatgaa ctgctagacc tgaaaacatc
     4681 aaacttcttc atttctctgc ttgctgataa ctgataaact gtttaaagaa taaatcattt
     4741 tcatgacaaa tagctacaac attgctgaac tatcagaaaa attgaaagtt gatcaataac
     4801 ttatggacaa tgaaaacata ccttattagt ggcaaaaaca atcgatgttt tacaaaacct
     4861 tcattcgaca ccgtttaaaa gtaaaaaaac acacagaact ttccaaaatt tatcagcatt
     4921 tcgagcacat tgaagttttt aatggcaact ctaccaacct ttctgaaacg taagacttct
     4981 cagtatcagt gtgagactat tgaacaagta gaggaaacgt ggtatgtcat tatgtggatt
     5041 tgaagttatt gcgcttgtta ttttgaaaaa gttcacaatt atttatccac aagttgaaaa
     5101 ttgtaaagct tacgacatta ctggctttct tataaaaagt ctaaaaagtt atagtagttg
     5161 aaaaatgaga tgagatgtcg actagaccat tcggcagttg cagaaaaatt atcgaaatgt
     5221 tttaaatttc tagaaaggta aaacgaagta tgttggttct catagagtac tgaatgagtg
     5281 ccaaagtgtc actcagactg tttcaaattt atgaagcacc ggaaaataac gctgtgaaaa
     5341 tttccataaa aaccagtatc tgcatatttc cttgaacgtt ctctcaccaa cgtcgcatac
     5401 cacattgctc taatttttgc tctttttttt tcaagcttca gtgagcacaa tgatgacttt
     5461 ttcaattacc tgtgataaac aatttcgtgg aatttttgta tttttttgaa gtttcctcgt
     5521 gttaacaaca atcaaaaacc gggtcatact ctagtgttgt atagtgggct tcaccataaa
     5581 ttacctgata aatcagatta gaggcacacg tcggaggagc acactgataa gaggtgataa
     5641 aaggagcacc gagtaacacg tgttttcagt tttccgcttc atttttcgca cagttttctt
     5701 catagtttcc cattaatttc ttacaaatta tttcaaataa atttgaaatt gcagtaatga
     5761 aatccatttt tgtcttcgcc gtgctggcag ctttggctct tggagcacaa tctgctccat
     5821 ctccatgtga atcatgcaaa tcggtaagta attatattca gagaactaaa tgttactact
     5881 ataaagtaaa caaatttcag atggttcaaa acttcattga tgcttctaag gatcgcatga
     5941 agatggctca actgaaggtt tctctttcaa tgctctgcgt aggtacatct catcaatctg
     6001 actgctcaaa gactcttgac aaacttgact tcatcgccta caaacttgct ccatacttgg
     6061 taggtttacg acattttttc agagagtact ctgaattttc aggctgacac ctccgctgta
     6121 tgctcaaagc tccaaatgtg cggtgaatct caattctcac cacttgctcg tcttgccatg
     6181 ctttacttga agaaatcaga ggccatcgta gctaatgata acattatggt ttgtcatttt
     6241 gggagattaa tttttaattt tcaattaatt tttatagaga caagaagttt gtgacgaatg
     6301 tcaggcatca actgcacaga tcggaaaact cgttggggat gaatttacta cctatgcagt
     6361 gagttaaatt ttgcaggaag aacggtaaaa ttatctcaag tttcaggtca aatctaccct
     6421 tcaacgcttt gtctgcaaga gcgccggaaa agcccacaag gcatgcaaca tctttgtctc
     6481 ctccgtcatt ccagatctta tgaccgagat gaaggatatg ttcaccgaaa aggaacttat
     6541 gtgctcaaac atgggactct gctctgctac atccaagcca gctgctcgtg aagctccgaa
     6601 acaaccagca tctgagatgt ggaagtctat gggaatggtg aagacaagca acggagagga
     6661 gcttatgtct tgcttcgagt gcacactttc cgctgatgct cttcttcagg aattcattga
     6721 taagagagta agtgcagtag aaaattgtgt tgcaaatttc aataaccact gcatgtattg
     6781 aaaagaaaat gtaaaagtat gttggaagca gttttaaaag gtttatattt tcagcaagga
     6841 acagctgatg acatccaaac agttgcctgc aataagatgg ttgccaactg gacagatgga
     6901 tgcaatgact ttgttcatat gtatatgtca actgtgcttt tcttgactta caatcaattt
     6961 gatggaagag gaatctgcac aatgatgcat tcatgcgaaa agaaggaaaa tggtaagtta
     7021 atcgatcatc tgaaatcctg taatttttca atttccagct ctcgtagaaa tggccatgtc
     7081 agagaaggtt atgttgggat gtgaaaactg taaagctgtc gagcacttct ttgctcaaaa
     7141 ccaagaggct cttcactctc acgctgttga cggactctac tcgaacgtct gtcaaaagct
     7201 tccaactgct cttggaacta tggtgagatg actcgtacca atggcagtga agtcacaaaa
     7261 ctacattctg agacacaaaa ctttagtgac accctataca tttgcaaaca catgttttaa
     7321 gtaccaatgt ttatttcgag accacctgaa aatcagaact tttcaatctt aggtctactg
     7381 tctagttcaa attgtatttt tgaatatttt tcgaaacatc tgcttaattc ttagcttaac
     7441 tttccatttt cagtgtgaag cctcaatcat ccgcctgtct cgcaagttct tcgcccgtac
     7501 cgccgacctc gccgcaagcg gagcaatgtg ctcgcaggtt tgtgcaaata tttaatttta
     7561 ctcggttttc acattttgac atcattcatt tgttttcaaa caactttttc tttcagatgt
     7621 gctagagcgt caatgctcca gagtttgaat caagaatttc gatattatct ccaatttcat
     7681 gttttcttat ttaaaatgaa catttcaatc gcaacttgtc gacttattag ttattgagaa
     7741 tcttttcaaa agtatcagcc aattatcaga aagtatacac ttttacatta atatataaat
     7801 gttggcaaat actcttttac tacctcatct tttactacga atcgtatctt ttactacggt
     7861 cgttttatca tttactattt ataaagtaga ctttccttcc gtccaatata ggctatgaca
     7921 acacgcataa gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
     7981 ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     8041 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8101 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8161 gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     8221 tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg gtgatccccc
     8281 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     8341 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     8401 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     8461 tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc ttcacctaga
     8521 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     8581 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     8641 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     8701 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     8761 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
     8821 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     8881 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     8941 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     9001 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9061 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9121 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9181 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9241 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9301 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9361 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9421 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9481 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9541 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9601 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc
     9661 tccttacgca tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt
     9721 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9781 acaataaccc tgataaatgc ttcaataata gcacgtgagg agggccacca tggccaagtt
     9841 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     9901 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     9961 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
    10021 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
    10081 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
    10141 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
    10201 ctgacacgtg ctaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10261 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10321 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10381 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10441 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    10501 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10561 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10621 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10681 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10741 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10801 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10861 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10921 cctatggaaa aacgccagca acgcggcctt tttacggttc ctgggctttt gctggccttt
    10981 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    11041 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11101 ggaagcggaa g
//
