LOCUS       Exported                 788 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    SP6-VEE-OKS-iM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        61..79
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     61..78
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcgagctcg
       61 atttaggtga cactatagat gggcggcgca tgagagaagc ccagaccaat tacctaccca
      121 aaatggagaa agttcacgtt gacatcgagg aagacagccc attcctcaga gctttgcagc
      181 ggagcttccc gcagtttgag gtagaagcca agcaggtcac tgataatgac catgctaatg
      241 ccagagcgtt ttcgcatctg gcttcaaaac tgatcgaaac ggaggtggac ccatccgaca
      301 cgatccttga cattggaagt gcgcccgccc gcagaatgta ttctaagcac aagtatcatt
      361 gtatctgtcc gatgagatgt gcggaagatc cggacagatt gtataagtat gcaactaagc
      421 tgaagaaaaa ctgtaaggaa ataactgata aggaattgga caagaaaatg aaggagctgg
      481 ccgccgtcat gagcgaccct gacctggaaa ctgagactat gtgcctccac gacgacgagt
      541 cgtgtcgcta cgaagggcaa gtcgctgttt accaggatgt atacgcggtt gacggaccga
      601 caagtctcta tcaccaagcc aataagggag ttagagtcgc ctactggata ggctttgaca
      661 ccaccccttt tatgtttaag aacttggctg gagcatatcc atcatactct accaactggg
      721 ccgacgaaac cgtgttaacg gctcgtaaca taggcctatg cagctctgac gttatggagc
      781 ggtcacgt
//
