LOCUS Exported 770 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION TALEN designed to target the human AAVS1/PPP1R12C locus. ACCESSION . VERSION . KEYWORDS AAVS1-TALEN-R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 770) AUTHORS Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D TITLE An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. JOURNAL Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. PUBMED 24931489 REFERENCE 2 (bases 1 to 770) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..770 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 258..274 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind complement(730..747) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(730..746) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg 61 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc 121 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa 181 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt caacgccact 241 gtgcatgaag ctcgtaattg ttatccgctc acaattaagg gcgacacaaa atttattcta 301 aatgataata aatactgata acatcttata gtttgtatta tattttgtat tatcgttgac 361 atgtataatt ttgatatcaa aaactgattt tccctttatt attttcgaga tttattttct 421 taattctctt taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa 481 tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg 541 cttttttctc atttataagg ttaaataatt ctcatatatc aagcaaagtg acaggcgccc 601 ttaaatattc tgacaaatgc tctttcccta aactcccccc ataaaaaaac ccgccgaagc 661 gggtttttac gttatttgcg gattaacgat tactcgttat cagaaccgcc cagggggccc 721 gagcttaaga ctggccgtcg ttttacaaca cagaaagagt ttgtagaaac //