LOCUS       Exported                 834 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    SP6-VEE-OKS-iG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        41..59
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     41..58
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ggggcggagc ctatggaaaa acgccagcaa cgcgagctcg atttaggtga cactatagat
       61 gggcggcgca tgagagaagc ccagaccaat tacctaccca aaatggagaa agttcacgtt
      121 gacatcgagg aagacagccc attcctcaga gctttgcagc ggagcttccc gcagtttgag
      181 gtagaagcca agcaggtcac tgataatgac catgctaatg ccagagcgtt ttcgcatctg
      241 gcttcaaaac tgatcgaaac ggaggtggac ccatccgaca cgatccttga cattggaagt
      301 gcgcccgccc gcagaatgta ttctaagcac aagtatcatt gtatctgtcc gatgagatgt
      361 gcggaagatc cggacagatt gtataagtat gcaactaagc tgaagaaaaa ctgtaaggaa
      421 ataactgata aggaattgga caagaaaatg aaggagctgg ccgccgtcat gagcgaccct
      481 gacctggaaa ctgagactat gtgcctccac gacgacgagt cgtgtcgcta cgaagggcaa
      541 gtcgctgttt accaggatgt atacgcggtt gacggaccga caagtctcta tcaccaagcc
      601 aataagggag ttagagtcgc ctactggata ggctttgaca ccaccccttt tatgtttaag
      661 aacttggctg gagcatatcc atcatactct accaactggg ccgacgaaac cgtgttaacg
      721 gctcgtaaca taggcctatg cagctctgac gttatggagc ggtcacgtag agggatgtcc
      781 attcttagaa agaagtattt gaaaccatcc aacaatgttc tattctctgt tggc
//
