LOCUS       Exported                 684 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    SP6-VEE-OKS-iG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 acctccccga gggcagcctg aagcggtgct gcgtcttggg cctacccccc acctccccag
       61 cctcctcctc accctgtgcc tcctccgacg tcacctccat catccgctcc tcccagacgt
      121 ctctggtcac ctgtgtaaat ggactccgga gcccccctct gacgggagat ctggggggcc
      181 cttccaagcg ggcccggcct ggccctgcat cgacggacag ccatgagggc agcttgcaac
      241 ttgaagcctg ccggaaggcg agcttcctga agcaggaacc cgcggatgag ttttcagagc
      301 tctttgggcc tcaccagcag ggcctgccgc ccccctatcc cctgtctcag ttgccgcctg
      361 gcccaagcct tggaggcctg gggctgggcc tggcaggcag ggtggtggcc gggcggcagg
      421 cgtgccgctg ggtggactgc tgtgcagcct atgagcagca ggaggagctg gtgcggcaca
      481 tcgagaagag ccacatcgac cagcgcaagg gcgaggactt cacctgcttc tgggctggct
      541 gcgtgcgccg ctacaagccc ttcaacgccc gctacaagct gctcatccac atgcgagtgc
      601 actcgggcga gaagcccaac aagtgcatgt ttgaaggctg cagcaaggcc ttctcacggc
      661 tggagaacct caagatccac ctga
//
