LOCUS       Exported                 775 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    SP6-VEE-OKS-iM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(438..497)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 accccgctcg ccatgctatt gccgccgggg gccagcagcc cgccgggcag cgtgtactta
       61 tccttcttca tgagcgtctt ggttttccgc cggggccggt atttataatc cgggtgctcc
      121 ttcatgtgca gcgctcgcag ccgcttagcc tcgtcgatga acggccgctt ctccgtctcc
      181 gacaaaagtt tccactcggc gcccaggcgc ttgctgatct ccgagttgtg catcttgggg
      241 ttctcctggg ccatcttgcg ccgctgcccg cgggaccaca ccatgaaggc attcatgggc
      301 cgcttgacgc ggtccgggct gtttttctgg ttgccgccgg ccgccgccgc ggtggagttg
      361 ccgccgccgc cccccgaagt ttgctgcggg cccggcggct tcagctccgt ctccatcatg
      421 ttgtacatgg gcgcgccagg accggggtta ctttcaacat cgccagcgag tttcaacaaa
      481 gcgtagttag tacattgtct agaaaaatgc ctcttcatgt gtaaggcgag gtggtccgac
      541 ctggaaaatg ctcggtcgca tttttggcac tggaacgggc ggtgccccgt gtgtttacgg
      601 tagtgcctgg tcagttcatc tgagcgggcg aatttccatc cacagccgtc ccagtcacag
      661 tggtaaggtt tctcacctgt gtgggttcgc aggtgtgcct tgagatggga actctttgtg
      721 taggttttgc cgcagcccgc gtaatcacaa gtgtgggtgg cggtcctttt ccggg
//
