LOCUS       Exported                 797 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Localization: Microtubules, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    mEmerald-MAP4-N-10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 797)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(129..380)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(171..192)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     520..539
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      181 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc
      241 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      301 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc gggtcttgaa gttcaccttg
      361 atgccgttct tctgcttgtc ggcggtgata tagaccttgt ggctgttgta gttgtactcc
      421 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      481 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      541 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      601 tgctgcttca tgtggtcggg gtagcgggcg aagcactgca cgccgtaggt caaggtggtc
      661 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
      721 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
      781 tcgccgtcca gctcgac
//
