LOCUS       Exported                 682 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Flp-Dependent EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Ef1a-fDIO EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      10..19
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(26..59)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(221..241)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(639..650)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccttgctcac catggtggcg ctagcgaagt tcctatactt tctagagaat aggaacttct
       61 tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagag aagttcctat
      121 accttttgaa gaataggaac ttcgaattcg atatcaagct tatcgataat caacctctgg
      181 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
      241 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
      301 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
      361 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
      421 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
      481 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
      541 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
      601 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
      661 ttcctccccg cggcctgctg cc
//
