LOCUS Exported 6131 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Non-integrating (episomal) expression of mouse c-Myc. ACCESSION . VERSION . KEYWORDS pCX-cMyc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6131) AUTHORS Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S TITLE Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. JOURNAL Science. 2008 Oct 9. ():. PUBMED 18845712 REFERENCE 2 (bases 1 to 6131) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6131 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 385..661 /label=chicken beta-actin promoter intron 662..1678 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1602..1624 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1686..1705 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind complement(3152..3171) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 3217..3272 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3271..3290) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(3633..3649) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3633..3649) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3646..3668) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3657..3673 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3681..3711) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3726..3747 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3806..4001 /label=SV40 promoter /note="SV40 early promoter" rep_origin 3852..3987 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3914..3933 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 4007..4141 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4044..4063) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4098..4117 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4209..4226) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4380..4968) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4460..4479) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5139..5999) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5762..5781 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6000..6104) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc 541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc 721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc 781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga 841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg 901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg 961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg 1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt 1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca 1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg 1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc 1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca 1741 ccatgcccct caacgtgaac ttcaccaaca ggaactatga cctcgactac gactccgtac 1801 agccctattt catctgcgac gaggaagaga atttctatca ccagcaacag cagagcgagc 1861 tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt cgagctgctt cccaccccgc 1921 ccctgtcccc gagccgccgc tccgggctct gctctccatc ctatgttgcg gtcgctacgt 1981 ccttctcccc aagggaagac gatgacggcg gcggtggcaa cttctccacc gccgatcagc 2041 tggagatgat gaccgagtta cttggaggag acatggtgaa ccagagcttc atctgcgatc 2101 ctgacgacga gaccttcatc aagaacatca tcatccagga ctgtatgtgg agcggtttct 2161 cagccgctgc caagctggtc tcggagaagc tggcctccta ccaggctgcg cgcaaagaca 2221 gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc cacctccagc ctgtacctgc 2281 aggacctcac cgccgccgcg tccgagtgca ttgacccctc agtggtcttt ccctacccgc 2341 tcaacgacag cagctcgccc aaatcctgta cctcgtccga ttccacggcc ttctctcctt 2401 cctcggactc gctgctgtcc tccgagtcct ccccacgggc cagccctgag cccctagtgc 2461 tgcatgagga gacaccgccc accaccagca gcgactctga agaagagcaa gaagatgagg 2521 aagaaattga tgtggtgtct gtggagaaga ggcaaacccc tgccaagagg tcggagtcgg 2581 gctcatctcc atcccgaggc cacagcaaac ctccgcacag cccactggtc ctcaagaggt 2641 gccacgtctc cactcaccag cacaactacg ccgcaccccc ctccacaagg aaggactatc 2701 cagctgccaa gagggccaag ttggacagtg gcagggtcct gaagcagatc agcaacaacc 2761 gcaagtgctc cagccccagg tcctcagaca cggaggaaaa cgacaagagg cggacacaca 2821 acgtcttgga acgtcagagg aggaacgagc tgaagcgcag cttttttgcc ctgcgtgacc 2881 agatccctga attggaaaac aacgaaaagg cccccaaggt agtgatcctc aaaaaagcca 2941 ccgcctacat cctgtccatt caagcagacg agcacaagct cacctctgaa aaggacttat 3001 tgaggaaacg acgagaacag ttgaaacaca aactcgaaca gcttcgaaac tctggtgcat 3061 aaaagggcga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg 3121 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg 3181 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 3241 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc 3301 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg 3361 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg 3421 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt 3481 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat 3541 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc 3601 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 3661 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 3721 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 3781 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc 3841 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 3901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 3961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc 4021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 4081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 4141 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 4201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 4261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 4321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 4381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 4501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 4561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 4621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 4681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 4741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 4801 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 4861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 4921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 4981 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 5041 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 5101 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 5161 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 5221 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 5281 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 5341 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 5401 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 5461 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 5521 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 5581 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 5641 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 5701 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 5761 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 5821 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 5881 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 5941 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 6001 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 6061 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 6121 gtgccacctg g //