LOCUS Exported 9895 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS FU-tet-o-hc-myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9895) AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K TITLE A high-efficiency system for the generation and study of human induced pluripotent stem cells. JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5. PUBMED 18786420 REFERENCE 2 (bases 1 to 9895) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9895 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2610..2880 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2913..2950 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2947..2971 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 3074..3083 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3089..3115 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3134..3160 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3179..3205 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3227..4543 /codon_start=1 /product="human c-Myc proto-oncogene" /label=c-Myc /translation="PLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPS EDIWKKFELLPAPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTEL LGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNP ARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSL LSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSP SAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRK CTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT AYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA" misc_feature 4587..5175 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4640..4660) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5058..5069) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5178..5194) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5179..5195) /label=pBluescriptKS /note="For pBluescript vector" LTR 5700..5880 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5906..5923) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5912..6136 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6182..6610 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6269..6288) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6479..6500 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6619..6639) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6624..6953 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6804..6939 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6866..6885 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7001..7048 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7067..7441 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7571..7692 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7608..7627) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7662..7681 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7741..7757) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7741..7757) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7754..7776) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7765..7781 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7789..7819) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7834..7855 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7972..7989) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8143..8731) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8223..8242) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8902..9762) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9525..9544 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9763..9867) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat 2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg 2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact 2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat 2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga 3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattga tcttcggcca 3061 gcgactagta gaagccacca tggatagcta tccttatgac gtgcctgact atgccagcct 3121 gatgctgatg agctatcctt atgacgtgcc tgactatgcc agcctgatgc tgatgagcta 3181 tccttatgac gtgcctgact atgccagcct gatgctgatg tctagacccc tcaacgttag 3241 cttcaccaac aggaactatg acctcgacta cgactcggtg cagccgtatt tctactgcga 3301 cgaggaggag aacttctacc agcagcagca gcagagcgag ctgcagcccc cggcgcccag 3361 cgaggatatc tggaagaaat tcgagctgct gcccgcgccg cccctgtccc ctagccgccg 3421 ctccgggctc tgctcgccct cctacgttgc ggtcacaccc ttctcccttc ggggagacaa 3481 cgacggcggt ggcgggagct tctccacggc cgaccagctg gagatggtga ccgagctgct 3541 gggaggagac atggtgaacc agagtttcat ctgcgacccg gacgacgaga ccttcatcaa 3601 aaacatcatc atccaggact gtatgtggag cggcttctcg gccgccgcca agctcgtctc 3661 agagaagctg gcctcctacc aggctgcgcg caaagacagc ggcagcccga accccgcccg 3721 cggccacagc gtctgctcca cctccagctt gtacctgcag gatctgagcg ccgccgcctc 3781 agagtgcatc gacccctcgg tggtcttccc ctaccctctc aacgacagca gctcgcccaa 3841 gtcctgcgcc tcgcaagact ccagcgcctt ctctccgtcc tcggattctc tgctctcctc 3901 gacggagtcc tccccgcagg gcagccccga gcccctggtg ctccatgagg agacaccgcc 3961 caccaccagc agcgactctg aggaggaaca agaagatgag gaagaaatcg atgttgtttc 4021 tgtggaaaag aggcaggctc ctggcaaaag gtcagagtct ggatcacctt ctgctggagg 4081 ccacagcaaa cctcctcaca gcccactggt cctcaagagg tgccacgtct ccacacatca 4141 gcacaactac gcagcgcctc cctccactcg gaaggactat cctgctgcca agagggtcaa 4201 gttggacagt gtcagagtcc tgagacagat cagcaacaac cgaaaatgca ccagccccag 4261 gtcctcggac accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag 4321 gaggaacgag ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa 4381 caatgaaaag gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt 4441 ccaagcagag gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca 4501 gttgaaacac aaacttgaac agctacggaa ctcttgtgcg taagtcgacg gatcctgaga 4561 attaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 4621 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 4681 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 4741 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 4801 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 4861 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 4921 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 4981 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5041 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 5101 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 5161 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca 5221 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 5281 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 5341 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 5401 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 5461 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca 5521 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct 5581 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga 5641 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg 5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 5881 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 5941 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 6001 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 6061 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 6121 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 6181 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 6241 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 6301 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 6361 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 6421 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 6481 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 6541 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 6601 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 6661 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 6721 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 6781 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 6841 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 6901 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 6961 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 7021 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 7081 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 7141 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 7201 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 7261 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 7321 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 7381 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 7441 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 7501 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 7561 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 7621 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 7681 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 7741 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7801 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8581 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 8941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 9541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 9601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac //