LOCUS       Exported                 902 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Expresses mVenus-fusion protein of RsLP-LOV domain (Rhodobacter 
            sphaeroides ATCC 17025 protein).
ACCESSION   .
VERSION     .
KEYWORDS    mV-RsLP-LOV_367
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Grusch M, Schelch K, Riedler R, Reichhart E, Differ C, Berger W, 
            Ingles-Prieto A, Janovjak H
  TITLE     Spatio-temporally precise activation of engineered receptor tyrosine
            kinases by light.
  JOURNAL   EMBO J. 2014 Jul 1. pii: e201387695.
  PUBMED    24986882
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(601..852)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(643..664)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgagctcgga tccgctttgt tagcagccgg atcaagcttt tatccgggac cccttctttc
       61 ccaggccctc accagggctg cagcagcttg tgccagatgt ctccgactgt ccatttcgag
      121 ccgagcagcc acagttccta ttctggcgag ctccccagtc agtgcccctg cgtgtccagc
      181 tgcggctgcc tcttcgctat ttccgcttct acccagctcg aactgagaac cgaggaagta
      241 gtcaggagcg tcgggtctgc caccgacagg gtgcaggaac agcagattgt caaatggttc
      301 atcgttggct ctgtaattgc ggaggaccac ctggagctcc cttccgagct tgagggcatc
      361 tctgatgtca gcccgtgcct gagcattttc gtcgcctctc tggagaaatc tgcagttgaa
      421 tcccaggatc tggccctcag tatagccagt cattctcaga aatggagggt tggcgagcac
      481 caggggttgc tctggcaggg acatgtcaac gagggtcagt gcgacccctg acctgtcaaa
      541 cacagctcta atcttctcaa actgcttctg atccatggca ccggatgatc ctgaactgcc
      601 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      661 catgtgatcg cgcttctcgt tggggtcttt gctcagtttg gactggtagc tcaggtagtg
      721 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      781 gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt
      841 cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      901 cc
//
