LOCUS       Exported                 779 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  Cre-Dependent Chloride Channel.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Ef1a-DIO SwiChRca-TS-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Berndt A, Lee SY, Ramakrishnan C, Deisseroth K
  TITLE     Structure-guided transformation of channelrhodopsin into a 
            light-activated chloride channel.
  JOURNAL   Science. 2014 Apr 25;344(6182):420-4. doi: 10.1126/science.1252367.
  PUBMED    24763591
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    70..103
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    154..187
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      192..201
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat
       61 atcgaattca taacttcgta tagcatacat tatacgaagt tattctttgc accattctaa
      121 agaataacag tgataatttc tgggttaagg caaataactt cgtataggat actttatacg
      181 aagttatgct agccaccatg gactatggcg gcgctttgtc tgccgtcgga ctcttccaaa
      241 cctcatacac tcttgagaac aatggttctg ttatttgcat cccgaacaac ggccagtgct
      301 tctgcttggc ttggcttaaa tccaacggaa caaatgccga gaagttggct gccaacattc
      361 tgcagtggat ttcttttgcg ctttcagcgc tctgcctgat gttctacggc taccagacct
      421 ggaagtctac ttgcggctgg gaggagattt acgtggccac gatctcgatg atcaagttca
      481 tcatcgagta tttccatagc tttgacgaac ctgcggtgat ctactcatcc aacggcaaca
      541 agaccaagtg gcttcgttac gcgagctggc tgctgaccgc ccctgtcatt cttatccgtc
      601 tgagcaacct tacgggtctg gcgaacgact ataacaagcg taccatgggt ctgctggtgt
      661 cagatatcgg cacgatcgtg tggggcacca cggccgcgct gtccaaggga tacgtccgtg
      721 tcattttctt cctgatgggc ctgtgctacg gcatctacac attcttcaac gcagccaag
//
