LOCUS Exported 6845 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDESTmycYBX1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6845) AUTHORS Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T TITLE Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. JOURNAL RNA. 2008 Oct 31. ():. PUBMED 18978028 REFERENCE 2 (bases 1 to 6845) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6845 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 15..318 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 319..522 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 473..493 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 519..543 /label=LNCX /note="Human CMV promoter, forward primer" CDS 639..668 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" protein_bind 678..702 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(1715..1739) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(1771..1790) /label=T7 /note="T7 promoter, forward primer" promoter complement(1772..1790) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1801..1818) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1801..1817) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1810..1832) /label=M13/pUC Forward /note="In lacZ gene" polyA_signal 2222..2356 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2259..2278) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2313..2332 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 2482..2937 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2569..2588) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2779..2800 /label=F1ori-F /note="F1 origin, forward primer" promoter 3056..3413 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3079..3099) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3264..3399 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3326..3345 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3464..4258 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3518..3537) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4128..4147 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4322..4370 /note="synthetic polyadenylation signal" primer_bind complement(4411..4430) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4530..4552 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4590..4608) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4676..4780 /gene="bla" /label=AmpR promoter CDS 4781..5641 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4999..5018) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5812..6400 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6301..6320 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6554..6571 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg 361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg 481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag 541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc 601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag 661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccatga 721 gcagcgaggc cgagacccag cagccgcccg ccgccccccc cgccgccccc gccctcagcg 781 ccgccgacac caagcccggc actacgggca gcggcgcagg gagcggtggc ccgggcggcc 841 tcacatcggc ggcgcctgcc ggcggggaca agaaggtcat cgcaacgaag gttttgggaa 901 cagtaaaatg gttcaatgta aggaacggat atggtttcat caacaggaat gacaccaagg 961 aagatgtatt tgtacaccag actgccataa agaagaataa ccccaggaag taccttcgca 1021 gtgtaggaga tggagagact gtggagtttg atgttgttga aggagaaaag ggtgcggagg 1081 cagcaaatgt tacaggtcct ggtggtgttc cagttcaagg cagtaaatat gcagcagacc 1141 gtaaccatta tagacgctat ccacgtcgta ggggtcctcc acgcaattac cagcaaaatt 1201 accagaatag tgagagtggg gaaaagaacg agggatcgga gagtgctccc gaaggccagg 1261 cccaacaacg ccggccctac cgcaggcgaa ggttcccacc ttactacatg cggagaccct 1321 atgggcgtcg accacagtat tccaaccctc ctgtgcaggg agaagtgatg gagggtgctg 1381 acaaccaggg tgcaggagaa caaggtagac cagtgaggca gaatatgtat cggggatata 1441 gaccacgatt ccgcaggggc cctcctcgcc aaagacagcc tagagaggac ggcaatgaag 1501 aagataaaga aaatcaagga gatgagaccc aaggtcagca gccacctcaa cgtcggtacc 1561 gccgcaactt caattaccga cgcagacgcc cagaaaaccc taaaccacaa gatggcaaag 1621 agacaaaagc agccgatcca ccagctgaga attcgtccgc tcccgaggct gagcagggcg 1681 gggctgagta agcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtt 1741 gatcgcgtgc atgcgacgtc atagctctct ccctatagtg agtcgtatta taagctaggc 1801 actggccgtc gttttacaac gtcgtgactg ggaaaactgc tagcttggga tctttgtgaa 1861 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc 1921 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctagctgcat atgcttgctg 1981 cttgagagtt ttgcttactg agtatgattt atgaaaatat tatacacagg agctagtgat 2041 tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc ccctcagtcc 2101 tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt tacttgcttt 2161 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 2221 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 2281 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 2341 ttatcatgtc tggatcgatc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 2401 tgcgtattgg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 2461 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 2521 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 2581 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 2641 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 2701 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 2761 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 2821 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 2881 agctgattta acaaatattt aacgcgaatt ttaacaaaat attaacgttt acaatttcgc 2941 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct 3001 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag 3061 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 3121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 3181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 3241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 3301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 3361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg 3421 attcttctga cacaacagtc tcgaacttaa ggctagagcc accatgattg aacaagatgg 3481 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3541 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3601 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 3661 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 3721 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 3781 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 3841 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 3901 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 3961 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4021 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4081 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4141 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4201 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4261 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg 4321 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc 4381 gataaggatc cgcgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag 4441 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 4501 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 4561 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 4621 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 4681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 4741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 4801 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 4861 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 4921 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 4981 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 5041 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 5101 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 5161 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 5221 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 5281 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 5341 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 5401 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 5461 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 5521 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 5581 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 5641 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 5701 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 5761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 5821 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 5881 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 5941 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 6001 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 6061 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 6121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 6181 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc 6241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 6301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 6361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 6421 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 6481 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 6541 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 6601 gcctctcccc gcgcgttggc cgattcatta atgcagagct tgcaattcgc gcgtttttca 6661 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 6721 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 6781 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtagta cgaggccctt 6841 tcact //