LOCUS Exported 7622 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDESTmycIGF2BP3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7622) AUTHORS Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, Ojo T, Hafner M, Zavolan M, Tuschl T TITLE Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs. JOURNAL RNA. 2008 Oct 31. ():. PUBMED 18978028 REFERENCE 2 (bases 1 to 7622) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7622 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 15..318 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 319..522 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 473..493 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 519..543 /label=LNCX /note="Human CMV promoter, forward primer" CDS 639..668 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" protein_bind 678..702 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2492..2516) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(2548..2567) /label=T7 /note="T7 promoter, forward primer" promoter complement(2549..2567) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2578..2595) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2578..2594) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2587..2609) /label=M13/pUC Forward /note="In lacZ gene" polyA_signal 2999..3133 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3036..3055) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3090..3109 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3259..3714 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3346..3365) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3556..3577 /label=F1ori-F /note="F1 origin, forward primer" promoter 3833..4190 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3856..3876) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4041..4176 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4103..4122 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4241..5035 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4295..4314) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4905..4924 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 5099..5147 /note="synthetic polyadenylation signal" primer_bind complement(5188..5207) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5307..5329 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5367..5385) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5453..5557 /gene="bla" /label=AmpR promoter CDS 5558..6418 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5776..5795) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6589..7177 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7078..7097 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7331..7348 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac 61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact 121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa 181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg 241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta 301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg 361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg 421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg 481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag 541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc 601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag 661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt 721 cagtcgacat gaacaaactg tatatcggaa acctcagcga gaacgccgcc ccctcggacc 781 tagaaagtat cttcaaggac gccaagatcc cggtgtcggg acccttcctg gtgaagactg 841 gctacgcgtt cgtggactgc ccggacgaga gctgggccct caaggccatc gaggcgcttt 901 caggtaaaat agaactgcac gggaaaccca tagaagttga gcactcggtc ccaaaaaggc 961 aaaggattcg gaaacttcag atacgaaata tcccgcctca tttacagtgg gaggtgctgg 1021 atagtttact agtccagtat ggagtggtgg agagctgtga gcaagtgaac actgactcgg 1081 aaactgcagt tgtaaatgta acctattcca gtaaggacca agctagacaa gcactagaca 1141 aactgaatgg atttcagtta gagaatttca ccttgaaagt agcctatatc cctgatgaaa 1201 tggccgccca gcaaaacccc ttgcagcagc cccgaggtcg ccgggggctt gggcagaggg 1261 gctcctcaag gcaggggtct ccaggatccg tatccaagca gaaaccatgt gatttgcctc 1321 tgcgcctgct ggttcccacc caatttgttg gagccatcat aggaaaagaa ggtgccacca 1381 ttcggaacat caccaaacag acccagtcta aaatcgatgt ccaccgtaaa gaaaatgcgg 1441 gggctgctga gaagtcgatt actatcctct ctactcctga aggcacctct gcggcttgta 1501 agtctattct ggagattatg cataaggaag ctcaagatat aaaattcaca gaagagatcc 1561 ccttgaagat tttagctcat aataactttg ttggacgtct tattggtaaa gaaggaagaa 1621 atcttaaaaa aattgagcaa gacacagaca ctaaaatcac gatatctcca ttgcaggaat 1681 tgacgctgta taatccagaa cgcactatta cagttaaagg caatgttgag acatgtgcca 1741 aagctgagga ggagatcatg aagaaaatca gggagtctta tgaaaatgat attgcttcta 1801 tgaatcttca agcacattta attcctggat taaatctgaa cgccttgggt ctgttcccac 1861 ccacttcagg gatgccacct cccacctcag ggcccccttc agccatgact cctccctacc 1921 cgcagtttga gcaatcagaa acggagactg ttcatctgtt tatcccagct ctatcagtcg 1981 gtgccatcat cggcaagcag ggccagcaca tcaagcagct ttctcgcttt gctggagctt 2041 caattaagat tgctccagcg gaagcaccag atgctaaagt gaggatggtg attatcactg 2101 gaccaccaga ggctcagttc aaggctcagg gaagaattta tggaaaaatt aaagaagaaa 2161 actttgttag tcctaaagaa gaggtgaaac ttgaagctca tatcagagtg ccatcctttg 2221 ctgctggcag agttattgga aaaggaggca aaacggtgaa tgaacttcag aatttgtcaa 2281 gtgcagaagt tgttgtccct cgtgaccaga cacctgatga gaatgaccaa gtggttgtca 2341 aaataactgg tcacttctat gcttgccagg ttgcccagag aaaaattcag gaaattctga 2401 ctcaggtaaa gcagcaccaa caacagaagg ctctgcaaag tggaccacct cagtcaagac 2461 ggaagtaagc ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat 2521 cgcgtgcatg cgacgtcata gctctctccc tatagtgagt cgtattataa gctaggcact 2581 ggccgtcgtt ttacaacgtc gtgactggga aaactgctag cttgggatct ttgtgaagga 2641 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa 2701 ggtaaatata aaatttttaa gtgtataatg tgttaaacta gctgcatatg cttgctgctt 2761 gagagttttg cttactgagt atgatttatg aaaatattat acacaggagc tagtgattct 2821 aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc tcagtcctca 2881 cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 2941 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 3001 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3061 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 3121 tcatgtctgg atcgatcctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 3181 gtattggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 3241 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3301 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 3361 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 3421 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 3481 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 3541 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 3601 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 3661 tgatttaaca aatatttaac gcgaatttta acaaaatatt aacgtttaca atttcgcctg 3721 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg 3781 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg 3841 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 3901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 4021 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 4081 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 4141 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt 4201 cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac aagatggatt 4261 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 4321 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 4381 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 4441 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 4501 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 4561 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 4621 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 4681 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 4741 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 4801 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 4861 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 4921 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 4981 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 5041 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa 5101 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat 5161 aaggatccgc gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 5221 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 5281 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 5341 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 5401 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 5461 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 5521 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 5581 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 5641 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 5701 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 5761 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 5821 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 5881 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 5941 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 6001 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 6061 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 6121 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 6181 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 6241 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 6301 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 6361 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 6421 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 6481 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 6541 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 6601 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 6661 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 6721 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 6781 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 6841 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 6901 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6961 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga 7021 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 7081 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 7141 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 7201 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 7261 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 7321 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 7381 tctccccgcg cgttggccga ttcattaatg cagagcttgc aattcgcgcg tttttcaata 7441 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 7501 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 7561 agaaaccatt attatcatga cattaaccta taaaaatagg cgtagtacga ggccctttca 7621 ct //