LOCUS       Exported                1073 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  AAV expression of CsChR-GFP under the Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Syn-CsChR-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(66..779)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(108..129)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     457..476
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     716..737
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      121 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
      181 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      241 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
      301 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
      361 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      421 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      481 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      541 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
      601 agggtgggcc agggcacggg cagcttgccg gtggtgcaaa tgaacttcag ggtcagcttg
      661 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      721 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcact
      781 gctactaccg gtgccgctcc ctcgtcgtgt tcctcgtcct ggtccacgta ggtgtccagt
      841 tccaccatgt cgccggccac gttgaccttg gtcttcttgg tgatgttgcc gtgcaccagg
      901 atgtgctcgt ggatcttcac gcggatgaag tggcccatca ggccccagag gttcttgctc
      961 agcagatcgc acacttcgtg ggcgattccg ctgctgtagg cggtgatctt gccgaatccc
     1021 tctgggccga ccatgaacag gatggggaac atgctccaag tgaagaagaa gtc
//
