LOCUS Exported 526 bp ds-DNA linear SYN 07-JUN-2021 DEFINITION AAV-mediated expression of Mac-GFP under the CAG promoter, in floxed/reversed (Cre-dependent) manner. ACCESSION . VERSION . KEYWORDS pAAV-CAG-FLEX-Mac-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 526) AUTHORS Chow BY, Han X, Dobry AS, Qian X, Chuong AS, Li M, Henninger MA, Belfort GM, Lin Y, Monahan PE, Boyden ES TITLE High-performance genetically targetable optical neural silencing by light-driven proton pumps. JOURNAL Nature. 2010 Jan 7. 463(7277):98-102. PUBMED 20054397 REFERENCE 2 (bases 1 to 526) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 7, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..526 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 29..48 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind complement(83..116) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind complement(179..212) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(271..292) /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 61 tttggcaaag aattggatcc ccataacttc gtataaagta tcctatacga agttatatca 121 aaataggaag accaatgctt caccatcgac ccgaattgcc aagcatcacc atcgacccat 181 aacttcgtat aatgtatgct atacgaagtt atactaggga tccccttact tgtacagctc 241 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg 301 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag 361 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct 421 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc 481 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttg //