LOCUS Exported 11100 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pTYF-mCMV/SYN-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11100) AUTHORS Liu B, Paton JF, Kasparov S TITLE Viral vectors based on bidirectional cell-specific mammalian promoters and transcriptional amplification strategy for use in vitro and in vivo. JOURNAL BMC Biotechnol. 2008 . 8():49. PUBMED 18485188 REFERENCE 2 (bases 1 to 11100) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11100 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 75..196 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(86..105) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 140..159 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(439..981) /codon_start=1 /product="C-terminal portion of the p65 subunit of mouse NF-kappa-B" /label=p65 /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED FSSIADMDFSALLSQISS" CDS complement(1045..1485) /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /label=GAL4 DNA binding domain /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" promoter complement(1522..1540) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter complement(1586..1624) /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 1783..2230 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 2281..2290 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2287..3003 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2287..3003 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2332..2353) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2593..2612) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2940..2961 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3004..3069 /label=MCS /note="multiple cloning site" primer_bind complement(3196..3216) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3614..3625) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3928..4152 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(4285..4304) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind complement(5325..5342) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5496..6084) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5576..5595) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6255..7115) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6878..6897 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7116..7220) /gene="bla" /label=AmpR promoter primer_bind 7288..7306 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(7357..7379) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 7465..7603 /label=bom /note="basis of mobility region from pBR322" primer_bind 7518..7537 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8807..8827 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 10448..10681 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." ORIGIN 1 ggcctaggga taacagggta atgatatctc tgaccgctag catcgtcgac ccgggatccg 61 cggactagtc gcgttaagat acattgatga gtttggacaa accacaacta gaatgcagtg 121 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 181 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 241 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga 301 tcatgaacag actgtgagga ctgaggggcc tgaaatgagc cttgggactg tgaatctaaa 361 atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat tggatctact 421 tattcaagat cggccgctgg agctgatctg actcagcaga gcagagaagt ccatgtccgc 481 aatggaggag aagtcttcat ctccggagag accattggga agccccgagg tccccagggg 541 tgtgggagct gggtcagggg gcctctggga ccctgtcacc aggcgagtta tagcttcagg 601 gtactccatc agcatgggct cagctgtgga gtgagacatg gacacaccct ggttcaggag 661 ctgctgaaac tctgagttgt ccacagatgc caggtctgtg aacactcctg ggtctgtgct 721 gttgccaagc aaggccccca agtcttcatc agcatcaaac tgcaggtgca gcagggcttc 781 cgacagcgtg ccttccccag cctgggtgct ctttggaaca ggtgcagaca gggactgggg 841 aggacccggg gttagaactg gggcaggagc tgggggctga gccagaggta ccatggctga 901 ggaagggacc atggtctggg caaggactgg ggcagaggac ggtgctaagg ccagggcctg 961 gtttgagatc tgcccagaag gaaacaccat ggtgagctct gcagaagctt ctagaattcc 1021 agcccgggcg gatcccaatt ccggcgatac agtcaactgt ctttgacctt tgttactact 1081 ctcttccgat gatgatgtcg cacttattct atgctgtctc aatgttagag gcatatcagt 1141 ctccactgaa gccaatctat ctgtgacggc atctttattc acattatctt gtacaaataa 1201 tcctgttaac aatgctttta tatcctgtaa agaatccatt ttcaaaatca tgtcaaggtc 1261 ttctcgagga aaaatcagta gaaatagctg ttccagtctt tctagccttg attccacttc 1321 tgtcagatgt gccctagtca gcggagacct tttggttttg ggagagtagc gacactccca 1381 gttgttcttc agacacttgg cgcacttcgg tttttctttg gagcacttga gctttttaag 1441 tcggcaaata tcgcatgctt gttcgataga agacagtagc ttcatctttc aggaggctag 1501 cttagatccc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcgct 1561 agcggatctg acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc 1621 taacgcgcta gccttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc 1681 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag actctagagg 1741 gacgcgtatc gatggcgcca gctgcagagg gccctgcgta tgagtgcaag tgggttttag 1801 gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa 1861 gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag 1921 gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc 1981 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg 2041 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc 2101 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg 2161 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct 2221 gagagcgcag tcgactctag agctgcaggc aagcttggta ccgagctcgg atccactagt 2281 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 2341 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 2401 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 2461 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 2521 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 2581 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 2641 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 2701 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 2761 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 2821 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 2881 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 2941 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 3001 aagtccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc 3061 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacgcg gccgctcccg 3121 ttatttgcgc tctgttcctg ttaatcaacc tctggattac aaaatttgtg aaagattgac 3181 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 3241 gtatcatgct attacttccc gtacggcttt cattttctcc tccttgtata aatcctggtt 3301 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 3361 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctatcaac tcctttccgg 3421 gactttcgct ttccccctcc ctattgccac ggcggaactc attgccgcct gccttgcccg 3481 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 3541 gacgtccttt ccatggctgc tcgcctgtgt tgccaactgg attctgcgcg ggacgtcctt 3601 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggt 3661 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 3721 cgcctatcga ttagggataa cagggtaatg gtacctttaa gaccaatgac ttacaaggca 3781 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 3841 cagggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 3901 cactgcttaa gcctcaataa agctcgactg tgccttctag ttgccagcca tctgttgttt 3961 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 4021 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 4081 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 4141 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 4201 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 4261 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 4321 tcgccggctg tgcgggagaa cggagttcta ttatgactca aatcagtctc cccaagcatt 4381 cggggatcag agtttttaag gataacttag tgtgtagggg gccagtgagt tggagatgaa 4441 agcgtaggga gtcgaaggtg tccttttgcg ccgagtcagt tcctgggtgg gggccacaag 4501 atcggatgag ccagtttatc aatccggggg tgccagctga tccatggagt gcagggtctg 4561 caaaatatct caagcactga ttgatcttag gttttacaat agtgatgtta ccccaggaac 4621 aatttgggga aggtcagaat cttgtagcct gtagctgcat gactcctaaa ccataatttc 4681 ttttttgttt tttttttttt atttttgaga cagggtctca ctctgtcacc taggctggag 4741 tgcagtggtg caatcacagc tcactgcagc ctcaacgtcg taagctcaag cgatcctccc 4801 acctcagcct gcctggtagc tgagactaca agcgacgccc cagttaattt ttgtattttt 4861 ggtagaggca gcgttttgcc gtgtggccct ggctggtctc gaactcctgg gctcaagtga 4921 tccagcctca gcctcccaaa gtgctgggac aaccggggcc agtcactgca cctggcccta 4981 aaccataatt tctaatcttt tggctaattt gttagtccta caaaggcagt ctagtcccca 5041 ggcaaaaagg gggtttgttt cgggaaaggg ctgttactgt ctttgtttca aactataaac 5101 taagttcctc ctaaacttag ttcggcctac acccaggaat gaacaaggag agcttggagg 5161 ttagaagcac gatggaattg gttaggtcag atctctttca ctgtctgagt tataattttg 5221 caatggtggt tcaaagactg cccgcttctg acaccagtcg ctgcattaat gaatcggcca 5281 acgcgcgggg agaggcggtt tgcgtattgg cgctcttccg cttcctcgct cactgactcg 5341 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 5401 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 5461 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 5521 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 5581 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 5641 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc 5701 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 5761 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 5821 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 5881 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 5941 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6001 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6061 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6121 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 6181 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 6241 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 6301 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 6361 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 6421 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 6481 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 6541 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 6601 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 6661 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 6721 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 6781 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 6841 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 6901 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 6961 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7021 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 7081 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 7141 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 7201 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 7261 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 7321 gaactgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 7381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 7441 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 7501 gtactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 7561 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 7621 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagcgcgg 7681 ggaggcagag attgcagtaa gctgagatcg cagcactgca ctccagcctg ggcgacagag 7741 taagactctg tctcaaaaat aaaataaata aatcaatcag atattccaat cttttccttt 7801 atttatttat ttattttcta ttttggaaac acagtccttc cttattccag aattacacat 7861 atattctatt tttctttata tgctccagtt ttttttagac cttcacctga aatgtgtgta 7921 tacaaaatct aggccagtcc agcagagcct aaaggtaaaa aataaaataa taaaaaataa 7981 ataaaatcta gctcactcct tcacatcaaa atggagatac agctgttagc attaaatacc 8041 aaataaccca tcttgtcctc aataatttta agcgcctctc tccaccacat ctaactcctg 8101 tcaaaggcat gtgccccttc cgggcgctct gctgtgctgc caaccaactg gcatgtggac 8161 tctgcagggt ccctaactgc caagccccac agtgtgccct gaggctgccc cttccttcta 8221 gcggctgccc ccactcggct ttgctttccc tagtttcagt tacttgcgtt cagccaaggt 8281 ctgaaactag gtgcgcacag agcggtaaga ctgcgagaga aagagaccag ctttacaggg 8341 ggtttatcac agtgcaccct gacagtcgtc agcctcacag ggggtttatc acattgcacc 8401 ctgacagtcg tcagcctcac agggggttta tcacagtgca cccttacaat cattccattt 8461 gattcacaat ttttttagtc tctactgtgc ctaacttgta agttaaattt gatcagaggt 8521 gtgttcccag aggggaaaac agtatataca gggttcagta ctatcgcatt tcagggtggc 8581 ccgagagctg catccggagt atctagatgg agttccgcgt tacataactt acggtaaatg 8641 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg actgatgttc 8701 ccatagtaac gccaataggg actttccatt gacgctaatg ggagtttgtt ttggcaccaa 8761 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt 8821 aggcgtgtac tctagaaggt ctatataagc agagctcgtt tagtgaaccg tttatactac 8881 ttatctggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 8941 acccactgct taagcctcaa taaagcttgc cttgagtgct caaagtagtg tgtgcccgtc 9001 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 9061 ctagcagtgg cgcccgaaca gggacttgaa agcgaaagta aagccagagg agatctctcg 9121 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag 9181 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcgg 9241 tattaagcgg gggagaatta gataaatggg aaaaaattcg gttaaggcca gggggaaaga 9301 aacaatataa actaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 9361 atcctggcct tttagagaca tcagaaggct gtagacaaat actgggacag ctacaaccat 9421 cccttcagac aggatcagaa gaacttagat cattatataa tacaatagca gtcctctatt 9481 gtgtgcatca aaggatagat gtaaaagaca ccaaggaagc cttagataag atagaggaag 9541 agcaaaacaa aagtaagaaa aaggcacagc aagcagcagc tgacacagga aacaacagcc 9601 aggtcagcca aaattaccct atagtgcaga acctccaggg gcaaatggta catcaggcca 9661 tatcacctag aactttaaat gcatgggtaa aagtagtaga agagaaggct ttcagcccag 9721 aagtaatacc catgttttca gcattatcag aaggagccac cccacaagat ttaaatacca 9781 tgctaaacac agtgggggga catcaagcag ccatgcaaat gttaaaagag accatcaatg 9841 aggaagctgc agaatgggat agattgcatc cagtgcatgc agggcctatt gcaccaggcc 9901 agatgagaga accaagggga agtgacatag caggaactac tagcaaatta agagaacaat 9961 ttggaaataa taaaacaata atctttaagc aatcctcagg aggggaccca gaaattgtaa 10021 cgcacagttt taattgtgga ggggaatttt tctactgtaa ttcaacacaa ctgtttaata 10081 gtacttggtt taatagtact tggagtactg aagggtcaaa taacactgaa ggaagtgaca 10141 caatcacact cccatgcaga ataaaacaat ttataaacat gtggcaggaa gtaggaaaag 10201 caatgtatgc ccctcccatc agtggacaaa ttagatgttc atcaaatatt actgggctgc 10261 tattaacaag agatggtggt aataacaaca atgggtccga gatcttcaga cctggaggag 10321 gcgatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac 10381 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag 10441 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcac 10501 ggtcaatgac gctgacggta caggccagac aattattgtc tgatatagtg cagcagcaga 10561 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca 10621 tcaaacagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc 10681 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta 10741 gttggagtaa taaatctctg gaacagattt ggaataacat gacctggatg gagtgggaca 10801 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc 10861 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcttgcggc cggaattcac 10921 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 10981 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 11041 ttactaaaaa ttcnaaaatt ttcgggttta ttacagggac agcagagatc cactttgggc //