LOCUS Exported 661 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven. ACCESSION . VERSION . KEYWORDS pL-CRISPR.SFFV.GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 661) AUTHORS Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL TITLE Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. JOURNAL Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. PUBMED 24952903 REFERENCE 2 (bases 1 to 661) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..661 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 126..145 /label=LKO.1 5' /note="Human U6 promoter, forward primer" ORIGIN 1 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa 61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta 121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata 181 tcttgtggaa aggacgaaac accggagacg gttgtaaatg agcacacaaa atacacatgc 241 taaaatatta tattctatga cctttataaa atcaaccaaa atcttctttt taataacttt 301 agtatcaata attagaattt ttatgttcct ttttgcaaac ttttaataaa aatgagcaaa 361 ataaaaaaac gctagtttta gtaactcgcg ttgttttctt cacctttaat aatagctact 421 ccaccacttg ttcctaagcg gtcagctcct gcttcaatca ttttttgagc atcttcaaat 481 gttctaactc caccagctgc tttaactaaa gcattgtctt taacaactga cttcattagt 541 ttaacatctt caaatgttgc acctgatttt gaaaatcctg ttgatgtttt aacaaattct 601 aatccagctt caacagctat ttcacaagct ttcatgattt cttcttttgt taataaacaa 661 t //