LOCUS       Exported                 754 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    ADAMTS5_pCSdest
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    125..149
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag
      121 gatcacaagt ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg
      181 gtaccgaatt cgcggccgcg aattcgccct tatcccccgc cccgttaact tcatagcaaa
      241 taacaaatac ccataaagtc ccagtcgcgc agcccctccc cgcgggcagc gcactatgct
      301 gctcgggtgg gcgtccctgc tgctgtgcgc gttccgcctg cccctggccg cggtcggccc
      361 cgccgcgaca cctgcccagg ataaagccgg gcagcctccg actgctgcag cagccgccca
      421 gccccgccgg cggcaggggg aggaggtgca ggagcgagcc gagcctcccg gccacccgca
      481 ccccctggcg cagcggcgca ggagcaaggg gctggtgcag aacatcgacc aactctactc
      541 cggcggcggc aaggtgggct acctcgtcta cgcgggcggc cggaggttcc tcttggacct
      601 ggagcgagat ggttcggtgg gcattgctgg cttcgtgccc gcaggaggcg ggacgagtgc
      661 gccctggcgc caccggagcc actgcttcta tcggggcaca gtggacgcta gtccccgctc
      721 tctggctgtc tttgacctct gtgggggtct cgac
//
