LOCUS Exported 779 bp ds-DNA linear SYN 28-JUN-2021 DEFINITION PLlacO-1:RBS(st7):gfp . ACCESSION . VERSION . KEYWORDS pHL1801 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 779) AUTHORS Adamson DN, Lim HN TITLE Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. JOURNAL Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. PUBMED 23878244 REFERENCE 2 (bases 1 to 779) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 28, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..779 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(18..734) /codon_start=1 /product="Aequoria victoria green fluorescent protein" /label=GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(61..81) /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" primer_bind 676..704 /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" protein_bind 763..779 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 ccctgtacga ttaaagctta tttgtatagt tcatccatgc catgtgtaat cccagcagct 61 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca 121 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta 181 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg 241 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta 301 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt 361 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag 421 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc 481 ttgaaaaagt catgctgttt catatgatct gggtatctcg caaagcattg aacaccataa 541 ccgaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat 601 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg 661 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt gaaaagttct 721 tctcctttac gcattttttt ttcctcctta cccgggagta tcttgttatc cgctcacaa //