LOCUS Exported 11370 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469. ACCESSION . VERSION . KEYWORDS pMZ381 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11370) AUTHORS Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M TITLE Implementation of the CRISPR-Cas9 system in fission yeast. JOURNAL Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. PUBMED 25352017 REFERENCE 2 (bases 1 to 11370) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11370 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin 539..1320 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" CDS 1930..2724 /codon_start=1 /gene="Schizosaccharomyces pombe ura4" /product="orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis" /label=S. pombe ura4 /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH" primer_bind 3350..3372 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3364..3381 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3365..3381 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_RNA complement(3431..3482) /label=sTRSV HHRz /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)" promoter complement(3587..3943) /label=rrk1 promoter/leader /note="promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014)" primer_bind complement(3980..3996) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3980..3996) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3993..4015) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4004..4020 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4028..4058) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4073..4094 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4211..4228) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4382..4970) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4462..4481) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5141..6001) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5764..5783 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6002..6106) /gene="bla" /label=AmpR promoter promoter 6135..6876 /label=adh1 promoter /note="promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase" CDS 6897..11000 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11013..11033 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 11049..11296 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(11065..11083) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind 11343..11361 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataatcg atggccaacc ttccaattca ttaaatcgag tcctccatca gatgatttac 241 aaggttttgc atagaatccc taagtaagag aagttgttag tgatttcttc aagtttacta 301 aaatttacat acaaatggat gatctcttct ccattgtttc ctttcttctt gcaaacgcgt 361 tttacaaaga gatgacatgc tattttctga ttattttttt ctatagtttt ctattttgta 421 cgtccttggg agtccgaaat gtaaaatcgg ggtatggtta gtagcggcag cgttcattat 481 ggtatataaa agataaaaag taggggcaag cgagacagaa tgggatacaa gggcatcgtc 541 tataattata gctaaaaatt gtattttaat ttgtattttt tgtaatttta tttctcattg 601 ttttacttaa aatgaatgcg aattagagaa aacttcaacg aaatgtcaaa ataagctcag 661 caaaatatac aattttaggg aaagcgatca gcaacttatt ctccgttgta gattcttttt 721 cttcataaat tccaacaatg taactgccaa ttgatttttc tctagatgct aataaattga 781 aactaatgaa tataataaca caggttttga taaattgtaa actcttactc aaaaaaagaa 841 tgttaaaact aatttaacct ttttataaag gctcatggcg ttaaagagga tagtaaaaat 901 tctacaattt tttaatttaa tgtcacaatt gtcttgcgaa tataattcta ttaattttcg 961 ctaactgctg ttttagtact attactttca atatgcctta tatgcaactt taattcatga 1021 agaatgcaat atttttgcaa tctttcttgt actgagcgct ttttatcatt tagtactcat 1081 tatttaattt ttcagtaaag ggcaataaat aaatttgtag aagatgcaat gtaatctcct 1141 ctatcctttt gctcatatgt ttatgagtat acctagtcta gaaaggcttg tatttaaaat 1201 attattcaat aaaaattcac aatttttacg acatgtgcta ccattcactt aacttcctga 1261 ttataaaatt ggttcgttta tactaattac ttaagtacct ttaactaaac aaaatgccta 1321 tatatatatt aatttacaat gagtgtcaga taagtcacta tgtccgagtg gttaaggagt 1381 tagactcgcc atcgatgagc ttagctacaa atcccactgg ctatatgtat gcatttgtgt 1441 taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa taggaatttg 1501 ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt atactcacgt 1561 ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa agataatcag 1621 ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc cttgctttaa 1681 attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt tagatagttc 1741 tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat aaattcttaa 1801 ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat gtcgtgtttt 1861 cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta aaggtttaaa 1921 agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga ggggatgaaa 1981 aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa cttgtcagtc 2041 gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat tggaccctat 2101 gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga tatggtagaa 2161 aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg caaattcgca 2221 gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat tgcttcttgg 2281 gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg cctcaaagaa 2341 gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc caaaggctct 2401 ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca taccgatttt 2461 tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta cataactatg 2521 tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata tcgtactcct 2581 gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg agtctatgga 2641 gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg gaaggcatat 2701 cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg gttggttatt 2761 gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca gaaggcattt 2821 agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa tattaactat 2881 tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt aaacatgctc 2941 ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa gagtgaattt 3001 ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt agatgaatgt 3061 aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt tggaacagaa 3121 taaattagat gtcaaaaagt ttcgtcaata tcacaagctc atcgcgatgg tatgcggtgt 3181 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg 3241 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 3301 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 3361 cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc tagaggatcc 3421 ccacggtacc cctgtttcgt cctcacggac tcatcagacc ggaaagcaca tccggtgaca 3481 gggcaccacc gactcggtgc cactcacttt tttcaagttg ataacggact agccttattt 3541 taacttgcta tttctagctc taaaaccaag gcatctgaac aatagattct tcggtacagg 3601 ttatgttttt tggcaacagc aactattcaa atcaactgaa agttttttag aattcaacaa 3661 cttccaatac tcaagtgtcc aagctagcaa actgtttaat aatcgtgacc caacattcct 3721 ttcatgcgca ccctcagcgt cacataaatc aacactttac aacacctatt caaagcttct 3781 atatgccttc gtcatttaaa tgtctaacat ttcaaaaaag tgtttgctaa tggtaaattc 3841 ttgagttttc ttggaagcac atatataacg agtgtgactg ttctacgctc cttgctgact 3901 agtcatcagt ctacgcatgc caactaccac caacataagc aaaaggtacc cggggtaccg 3961 agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 4021 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 4081 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 4141 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 4201 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 4261 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 4321 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 4561 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 4621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 4681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 4741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 4801 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 4861 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4921 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4981 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 5041 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 5101 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 5161 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 5221 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 5281 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 5341 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 5401 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 5461 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 5521 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5581 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5641 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5701 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 5761 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5821 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5881 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5941 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 6001 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 6061 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 6121 aagtgccacc tgacgcccta caacaactaa gaaaatggct atcatgcgga agctggtgag 6181 aagaacagca tcgggacaag ggaaggaaga acaaagacaa agaaaacaaa agaaagcaat 6241 tgaaaacaaa acaaaacaat tttcattcct tctcttatca ttccttttct tttcttttct 6301 ctcattcaac gcactccatc gtatccgtat tcctcttatt ttttctcttt ctctatatcc 6361 atttctttct ctctaggtgt gtcctctctc tctcttcaat ttctctactc cgcattccaa 6421 cgcatccttc ccccaacctc ccatttcctc cttacggccc gatagcgatc gtctttccct 6481 cgctatcact cgctaccggc ccctcctctg caccgtaacc tcctacgtat ttaccatatc 6541 ataaagtttt ttccgacgct tatcgctgac cccctgtcgc cctcctattg gcttccggat 6601 tatcttcttg tccataaggt gatccatgct tcctgaagat tcccgaaatg tgtccacttt 6661 ggcggggaat cattccatcc acttctttct ctctcgcttt cctcattcgg cgctcccctt 6721 ccgcgtctca ttggtcttcc gctccgtttt tgctttgccg atgttacttg gggagaggtg 6781 cgataatcct ttcgcaaaaa ctcggtttga cgcctcccat ggtataaata gtgggtggtg 6841 gacaggtgcc ttcgcttttc tttaagcaag agaattgctg caggtcgact ctagagatgg 6901 acaagaagta ctccattggg ctcgatatcg gcacaaacag cgtcggttgg gccgtcatta 6961 cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca 7021 gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca 7081 cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc 7141 tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg 7201 aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata 7261 tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc 7321 ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga 7381 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg 7441 acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca 7501 acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc 7561 tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta 7621 tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg 7681 ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga 7741 tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc 7801 tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga 7861 tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc 7921 aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat 7981 acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa 8041 aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac 8101 agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta 8161 tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga 8221 aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga aattccagat 8281 tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc ctggaacttc gaggaagtcg 8341 tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc 8401 tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata 8461 acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg 8521 gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga 8581 aacagctcaa agaagactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg 8641 gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta 8701 aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca 8761 cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc 8821 tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc 8881 tgtcaagaaa actgatcaat gggatccgag ataagcagtc tggaaagaca atcctggatt 8941 ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc 9001 tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac agtcttcacg 9061 agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta 9121 aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg 9181 agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga 9241 agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg 9301 aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca 9361 tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatcatatcg 9421 tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata 9481 aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt 9541 attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta 9601 aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg 9661 ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca 9721 agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc 9781 tggtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc 9841 accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc 9901 ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga 9961 tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata 10021 ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac 10081 ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga 10141 cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga 10201 ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac 10261 gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca 10321 gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg 10381 aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc 10441 tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact 10501 ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga 10561 aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact 10621 atgaaaagct caaagggtct cccgaagata atgagcagaa gcagctgttc gtggaacaac 10681 acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc 10741 tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca 10801 tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg 10861 cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg 10921 tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc 10981 tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgatctc 11041 ttctcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc 11101 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat 11161 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag 11221 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc 11281 gaaggcttta atttgcgatc cccgtctaag aaaccattat tatcatgaca ttaacctata 11341 aaaataggcg tatcacgagg ccctttcgtc //