LOCUS       Exported                 662 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  lentiviral expression of constitutively active human Akt3 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    pHRIG-Akt3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H
  TITLE     Akt isoforms differentially protect against stroke-induced neuronal 
            injury by regulating mTOR activities.
  JOURNAL   J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 
            10.1038/jcbfm.2013.132. Epub 2013 Aug 14.
  PUBMED    23942361
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      117..126
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             123..164
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgactctaga ggatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggcca
      121 ccatggggag tagcaagagc aagcctaagg accccagcca gcgcagcgat ggagaggaag
      181 agatggatgc ctctacaacc catcataaaa gaaagacaat gaatgatttt gactatttga
      241 aactactagg taaaggcact tttgggaaag ttattttggt tcgagagaag gcaagtggaa
      301 aatactatgc tatgaagatt ctgaagaaag aagtcattat tgcaaaggat gaagtggcac
      361 acactctaac tgaaagcaga gtattaaaga acactagaca tcccttttta acatccttga
      421 aatattcctt ccagacaaaa gaccgtttgt gttttgtgat ggaatatgtt aatgggggcg
      481 agctgttttt ccatttgtcg agagagcggg tgttctctga ggaccgcaca cgtttctatg
      541 gtgcagaaat tgtctctgcc ttggactatc tacattccgg aaagattgtg taccgtgatc
      601 tcaagttgga gaatctaatg ctggacaaag atggccacat aaaaattaca gattttggac
      661 tt
//
