LOCUS       Exported                  20 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse 
            beta-Catenin. Expresses Cas9 from CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pX330 Ctnnb1.2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20)
  AUTHORS   Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
            W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, 
            Jacks T
  TITLE     CRISPR-mediated direct mutation of cancer genes in the mouse liver.
  JOURNAL   Nature. 2014 Aug 6. doi: 10.1038/nature13589.
  PUBMED    25119044
REFERENCE   2  (bases 1 to 20)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..20
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 agctccttcc ctgagtggca
//
