LOCUS Exported 10031 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS TetO-FUW-Klf4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10031) AUTHORS Brambrink T, Foreman R, Welstead GG, Lengner CJ, Wernig M, Suh H, Jaenisch R TITLE Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. JOURNAL Cell Stem Cell. 2008 Feb 7. 2(2):151-9. PUBMED 18371436 REFERENCE 2 (bases 1 to 10031) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10031 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 7..386 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 388..586 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 541..561 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 604..784 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 831..956 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1449..1682 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1867..1911 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2209..2326 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2383..2653 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2686..2723 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2720..2744 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 4492..5080 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4545..4565) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4963..4974) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5083..5099) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5084..5100) /label=pBluescriptKS /note="For pBluescript vector" LTR 5605..5785 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5811..5828) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5817..6041 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6087..6515 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6174..6193) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6384..6405 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6524..6544) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6529..6858 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6709..6844 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6771..6790 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6906..6953 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6972..7346 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7476..7597 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7513..7532) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7567..7586 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7646..7662) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7646..7662) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7659..7681) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7670..7686 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7694..7724) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7739..7760 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7877..7894) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8048..8636) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8128..8147) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8807..9667) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9430..9449 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9668..9772) /gene="bla" /label=AmpR promoter primer_bind complement(9847..9866) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg 2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa 2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag 2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag 2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt 2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg 2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt 2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc 2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat 2821 tcggccatta cggccggttt gggtacccct ctctcttctt cggactccgg aggaccttct 2881 gggcccccac attaatgagg cagccacctg gcgagtctga catggctgtc agcgacgctc 2941 tgctcccgtc cttctccacg ttcgcgtccg gcccggcggg aagggagaag acactgcgtc 3001 cagcaggtgc cccgactaac cgttggcgtg aggaactctc tcacatgaag cgacttcccc 3061 cacttcccgg ccgcccctac gacctggcgg cgacggtggc cacagacctg gagagtggcg 3121 gagctggtgc agcttgcagc agtaacaacc cggccctcct agcccggagg gagaccgagg 3181 agttcaacga cctcctggac ctagacttta tcctttccaa ctcgctaacc caccaggaat 3241 cggtggccgc caccgtgacc acctcggcgt cagcttcatc ctcgtcttcc ccagcgagca 3301 gcggccctgc cagcgcgccc tccacctgca gcttcagcta tccgatccgg gccgggggtg 3361 acccgggcgt ggctgccagc aacacaggtg gagggctcct ctacagccga gaatctgcgc 3421 cacctcccac ggcccccttc aacctggcgg acatcaatga cgtgagcccc tcgggcggct 3481 tcgtggctga gctcctgcgg ccggagttgg acccagtata cattccgcca cagcagcctc 3541 agccgccagg tggcgggctg atgggcaagt ttgtgctgaa ggcgtctctg accacccctg 3601 gcagcgagta cagcagccct tcggtcatca gtgttagcaa aggaagccca gacggcagcc 3661 accccgtggt agtggcgccc tacagcggtg gcccgccgcg catgtgcccc aagattaagc 3721 aagaggcggt cccgtcctgc acggtcagcc ggtccctaga ggcccatttg agcgctggac 3781 cccagctcag caacggccac cggcccaaca cacacgactt ccccctgggg cggcagctcc 3841 ccaccaggac tacccctaca ctgagtcccg aggaactgct gaacagcagg gactgtcacc 3901 ctggcctgcc tcttccccca ggattccatc cccatccggg gcccaactac cctcctttcc 3961 tgccagacca gatgcagtca caagtcccct ctctccatta tcaagagctc atgccaccgg 4021 gttcctgcct gccagaggag cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa 4081 gaacagccac ccacacttgt gactatgcag gctgtggcaa aacctatacc aagagttctc 4141 atctcaaggc acacctgcga actcacacag gcgagaaacc ttaccactgt gactgggacg 4201 gctgtgggtg gaaattcgcc cgctccgatg aactgaccag gcactaccgc aaacacacag 4261 ggcaccggcc ctttcagtgc cagaagtgcg acagggcctt ttccaggtcg gaccaccttg 4321 ccttacacat gaagaggcac ttttaaatcc cacgtagtgg atgtgaccca cactgccagg 4381 agagagagtt cagtattttt ttttctaacc tttcacactg tcttcccacg agggccgcct 4441 cggccctcga aacctcgagg gcgcgccgaa ttcgatatca agcttatcga taatcaacct 4501 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 4561 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 4621 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 4681 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 4741 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 4801 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 4861 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt 4921 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 4981 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 5041 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga 5101 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 5161 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 5221 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 5281 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac 5341 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat 5401 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa 5461 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac 5521 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc 5581 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag 5641 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 5701 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 5761 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt 5821 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 5881 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 5941 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 6001 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 6061 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 6121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 6181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 6241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 6301 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 6361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 6421 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 6481 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 6541 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 6601 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 6661 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 6721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 6781 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga 6841 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag 6901 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga 6961 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg 7021 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg 7081 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg 7141 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg 7201 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga 7261 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc 7321 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct 7381 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc 7441 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg 7501 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 7561 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct 7621 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 7681 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 7741 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 7801 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 7861 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 7921 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 7981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 8041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 8101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 8161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 8221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 8281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 8341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 8401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 8461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 8521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 8581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 8641 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 8701 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 8761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 8821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 8881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 8941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 9001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 9061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 9121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 9181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 9241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 9301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 9361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 9421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 9481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 9541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 9601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 9661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 9721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 9781 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac 9841 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt 9901 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac 9961 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg 10021 ggccagatat a //