LOCUS Exported 9363 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS FUW-SO SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9363) AUTHORS Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, Jaenisch R TITLE Reprogramming of murine and human somatic cells using a single polycistronic vector. JOURNAL Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. PUBMED 19109433 REFERENCE 2 (bases 1 to 9363) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9363 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1686..1919 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2104..2148 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2446..2563 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" primer_bind 2622..2638 /label=KS primer /note="common sequencing primer, one of multiple similar variants" protein_bind 2680..2713 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 2763..3974 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 3811..3830 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" CDS 4990..5055 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /label=F2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" protein_bind 6163..6196 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 6252..6840 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6305..6325) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6723..6734) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6843..6859) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 7369..7549 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" rep_origin complement(7611..8199) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7691..7710) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8370..9230) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8993..9012 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9231..9335) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga 1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat 2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat 2701 tatacgaagt tatattaagg gttccaagct taagcggcct aacccgtgtc ggctccagat 2761 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc 2821 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac 2881 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt 2941 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga 3001 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg 3061 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg 3121 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg 3181 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc 3241 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag 3301 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt 3361 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc 3421 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc 3481 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca 3541 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg 3601 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg 3661 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga 3721 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt 3781 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg 3841 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt 3901 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg 3961 cttttttgtt agacgaagct tgggctgcag gtcgactcta gagaattcgc caccatgtat 4021 aacatgatgg agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc 4081 ggaggaggca acgccacggc ggcggcgacc ggcggcaacc agaagaacag cccggaccgc 4141 gtcaagaggc ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc 4201 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa 4261 cttttgtccg agaccgagaa gcggccgttc atcgacgagg ccaagcggct gcgcgctctg 4321 cacatgaagg agcacccgga ttataaatac cggccgcggc ggaaaaccaa gacgctcatg 4381 aagaaggata agtacacgct tcccggaggc ttgctggccc ccggcgggaa cagcatggcg 4441 agcggggttg gggtgggcgc cggcctgggt gcgggcgtga accagcgcat ggacagctac 4501 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggagca gctgggctac 4561 ccgcagcacc cgggcctcaa cgctcacggc gcggcacaga tgcaaccgat gcaccgctac 4621 gacgtcagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg 4681 cccacctaca gcatgtccta ctcgcagcag ggcacccccg gtatggcgct gggctccatg 4741 ggctctgtgg tcaagtccga ggccagctcc agcccccccg tggttacctc ttcctcccac 4801 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta cctccccggc 4861 gccgaggtgc cggagcccgc tgcgcccagt agactgcaca tggcccagca ctaccagagc 4921 ggcccggtgc ccggcacggc cattaacggc acactgcccc tgtcgcacat ggctagcggc 4981 agcggcccgg tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtcgag 5041 tccaaccctg ggcccgcatg caccatggct ggacacctgg cttcagactt cgccttctca 5101 cccccaccag gtgggggtga tgggtcagca gggctggagc cgggctgggt ggatcctcga 5161 acctggctaa gcttccaagg gcctccaggt gggcctggaa tcggaccagg ctcagaggta 5221 ttggggatct ccccatgtcc gcccgcatac gagttctgcg gagggatggc atactgtgga 5281 cctcaggttg gactgggcct agtcccccaa gttggcgtgg agactttgca gcctgagggc 5341 caggcaggag cacgagtgga aagcaactca gagggaacct cctctgagcc ctgtgccgac 5401 cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc ccgaggagtc ccaggacatg 5461 aaagccctgc agaaggagct agaacagttt gccaagctgc tgaagcagaa gaggatcacc 5521 ttggggtaca cccaggccga cgtggggctc accctgggcg ttctctttgg aaaggtgttc 5581 agccagacca ccatctgtcg cttcgaggcc ttgcagctca gccttaagaa catgtgtaag 5641 ctgcggcccc tgctggagaa gtgggtggag gaagccgaca acaatgagaa ccttcaggag 5701 atatgcaaat cggagaccct ggtgcaggcc cggaagagaa agcgaactag cattgagaac 5761 cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc cgaagccctc cctacagcag 5821 atcactcaca tcgccaatca gcttgggcta gagaaggatg tggttcgagt atggttctgt 5881 aaccggcgcc agaagggcaa aagatcaagt attgagtatt cccaacgaga agagtatgag 5941 gctacaggga cacctttccc agggggggct gtatcctttc ctctgccccc aggtccccac 6001 tttggcaccc caggctatgg aagcccccac ttcaccacac tctactcagt cccttttcct 6061 gagggcgagg cctttccctc tgttcccgtc actgctctgg gctctcccat gcattcaaac 6121 tgagcatgcc tcgagggcgc gccgaattcg tcgagggacc taataacttc gtatagcata 6181 cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca 6241 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 6301 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 6361 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 6421 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 6481 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 6541 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 6601 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 6661 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 6721 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 6781 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 6841 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat 6901 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt 6961 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 7021 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 7081 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac 7141 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca 7201 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct 7261 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc 7321 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg 7381 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 7441 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 7501 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca 7561 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 7621 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 7681 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 7741 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 7801 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 7861 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 7921 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 7981 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 8041 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 8101 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 8161 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 8221 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 8281 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 8341 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 8401 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 8461 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 8521 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 8581 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 8641 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 8701 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 8761 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 8821 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 8881 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 8941 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 9001 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 9061 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 9121 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 9181 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 9241 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 9301 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 9361 gac //