LOCUS Exported 665 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Level 2 construct. ACCESSION . VERSION . KEYWORDS pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 665) AUTHORS Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V TITLE Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. JOURNAL Plant Methods. 2013 Oct 11;9(1):39. PUBMED 24112467 REFERENCE 2 (bases 1 to 665) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..665 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 38..112 /label=AtU6 promoter /note="consensus promoter from the three Arabidopsis U6 snRNA genes (Waibel and Filipowicz, 1990; Nekrasov et al., 2013)" misc_RNA 133..208 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" protein_bind complement(220..244) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 313..337 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 gttactagat cgacgctact agaattcgag ctcggagtga tcaaaagtcc cacatcgatc 61 aggtgatata tagcagctta gtttatataa tgatagagtc gacatagcga ttgccgttaa 121 tttgagagtc cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca 181 acttgaaaaa gtggcaccga gtcggtgctt tttttctaga cccagctttc ttgtacaaag 241 ttggcattac gctttacgag gatgcacatg tgaccgaggg acacgaagtg atccgtttaa 301 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta 361 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc 421 cagccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc 481 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg 541 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa 601 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa 661 gacga //