LOCUS       Exported                 573 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Lentiviral vector for use as a standard for titering experiments 
            when trying to compare vectors with different selection markers or 
            to determine appropriate antibiotic conditions..
ACCESSION   .
VERSION     .
KEYWORDS    pRosetta
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 573)
  TITLE     David Root plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(5..70)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(107..502)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(503..559)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tagcgggccc tgggttggac tccacgtctc ccgccaactt gagaaggtca aaattcaaag
       61 tctgtttcac aattttctgt ttgtgtctgg cttcgccaga tccctggccc tcccacacat
      121 aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca
      181 ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc
      241 tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg
      301 cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata
      361 cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt
      421 cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt
      481 cttgagacaa aggcttggcc ataggtccag ggttctcctc cacgtctcca gcctgcttca
      541 gcaggctgaa gttagtagct ccgcttccca ggg
//
