LOCUS       Exported                 726 bp ds-DNA     linear   SYN 02-JUL-2021
DEFINITION  Expresses eNpHR 3.0-EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    pRVdG-4Halo3Y
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 726)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(127..378)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(169..190)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     518..537
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agtaagtctc aggttgagaa gtgttgccag ttgttcttct gatctaatgt ttttttctcg
       61 actgaaaagc ttagatgacc cagcacttta taacccgggt cattacacct cgttctcgta
      121 gcagaacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      181 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
      241 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      301 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      361 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      421 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      481 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      541 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      601 ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg ccgtagccga aggtggtcac
      661 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
      721 gccgta
//
