LOCUS       Exported                 737 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Expresses EGFP-P2A-B19G.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-synP-FLEX-EGFP-B19G
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 737)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    411..444
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    495..528
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
ORIGIN
        1 tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg
       61 catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca
      121 gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc
      181 agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac
      241 cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag gcgcgagata
      301 ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct gcctcagtct
      361 gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agtcgaattc ataacttcgt
      421 atagcataca ttatacgaag ttattctttg caccattcta aagaataaca gtgataattt
      481 ctgggttaag gcaaataact tcgtatagga tactttatac gaagttatgc tagcttacag
      541 tctggtctca cccccactct tgtgtgattc ccatgaagat atgatcttcc cgctttgggg
      601 agtgactgac acctccctcc ctgtccctct gagattgtgt tgcgtaggtt ctgatcgatt
      661 gactcttcta caacatgtca tcaggaaaat tatcaacatc aaggcagtca gggcccctgc
      721 actcagtaat acatact
//
